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KLHL31 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL31 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G122484-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHL31 (NM_001003760), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BKLHD6 | BA345L23.2 | Kelch-like 31 (Drosophila) | Kelch-like protein KLHL | KLHL | KLHL31 | KBTBD1 | Kelch-like protein 31
Origin Species
Human
NCBI GenBank
NM_001003760
SwissProt
Q9H511

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPKKKIVKK NKGDINEMTI IVEDSPLNKL NALNGLLEGG NGLSCISSEL TDASYGPNLL
61EGLSKMRQEN FLCDLVIGTK TKSFDVHKSV MASCSEYFYN ILKKDPSIQR VDLNDISPLG
121LATVIAYAYT GKLTLSLYTI GSIISAAVYL QIHTLVKMCS DFLIREMSVE NCMYVVNIAE
181TYSLKNAKAA AQKFIRDNFL EFAESDQFMK LTFEQINELL IDDDLQLPSE IVAFQIAMKW
241LEFDQKRVKY AADLLSNIRF GTISAQDLVN YVQSVPRMMQ DADCHRLLVD AMNYHLLPYH
301QNTLQSRRTR IRGGCRVLVT VGGRPGLTEK SLSRDILYRD PENGWSKLTE MPAKSFNQCV
361AVMDGFLYVA GGEDQNDARN QAKHAVSNFC RYDPRFNTWI HLASMNQKRT HFSLSVFNGL
421VYAAGGRNAE GSLASLECYV PSTNQWQPKT PLEVARCCHA SAVADGRVLV TGGYIANAYS
481RSVCAYDPAS DSWQELPNLS TPRGWHCAVT LSDRVYVMGG SQLGPRGERV DVLTVECYSP
541ATGQWSYAAP LQVGVSTAGV SALHGRAYLV GGWNEGEKKY KKCIQCFSPE LNEWTEDDEL
601PEATVGVSCC TLSMPNNVTR ESRASSVSSV PVSI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

634 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.