LSBio
KLHL30 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL30 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KLHL30 antibodies →
$725.00each
Catalog Number: LS-G103601-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHL30 (NM_198582), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KLHL30 | Kelch-like protein 30 | Kelch-like 30 (Drosophila)
Origin Species
Human
NCBI GenBank
NM_198582
SwissProt
Q0D2K2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVRNVDDLDF HLPSHAQDML DGLQRLRSQP KLADVTLLVG GRELPCHRGL LALSSPYFHA
61MFAGDFAESF SARVELRDVE PAVVGQLVDF VYTGRLTITQ GNVEALTRTA ARLHFPSVQK
121VCGRYLQQQL DAANCLGICE FGEQQGLLGV AAKAWAFLRE NFEAVAREDE FLQLPRERLV
181TCLAGDLLQV QPEQSRLEAL MRWVRHDPQA RAAHLPELLS LVHLDAVPRP CVQQLLASEP
241LIQESEACRA ALSQGHDGAP LALQQKLEEV LVVVGGQALE EEEAGEEPTP GLGNFAFYNS
301KAKRWMALPD FPDYHKWGFS LAALNNNIYV TGGSRGTKTD TWSTTQAWCF PLKEASWKPV
361APMLKPRTNH ASAALNGEIY VIGGTTLDVV EVESYDPYTD SWTPVSPALK YVSNFSAAGC
421RGRLYLVGSS ACKYNALALQ CYNPVTDAWS VIASPFLPKY LSSPRCAALH GELYLIGDNT
481KKVYVYDPGA NLWQKVQSQH SLHENGALVP LGDALYVTGG RWQGMEGDYH VEMEAYDTVR
541DTWTRHGALP RLWLYHGAST VFLDVSKWTQ PSGPTQEH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

578 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.