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KLHL29 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL29 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G128381-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHL29 (NM_052920), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KBTBD9 | KIAA1921 | Kelch-like protein 29 | KLHL29 | Kelch-like 29 (Drosophila)
Origin Species
Human
NCBI GenBank
AK024168
SwissProt
Q96CT2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSRHHSRFER DYRVGWDRRE WSVNGTHGTT SICSVTSGAG GGTASSLSVR PGLLPLPVVP
61SRLPTPATAP APCTTGSSEA ITSLVASSAS AVTTKAPGIS KGDSQSQGLA TSIRWGQTPI
121NQSTPWDTDE PPSKQMRESD NPGTGPWVTT VAAGNQPTLI AHSYGVAQPP TFSPAVNVQA
181PVIGVTPSLP PHVGPQLPLM PGHYSLPQPP SQPLSSVVVN MPAQALYASP QPLAVSTLPG
241VGQVARPGPT AVGNGHMAGP LLPPPPPAQP SATLPSGAPA TNGPPTTDSA HGLQMLRTIG
301VGKYEFTDPG HPREMLKELN QQRRAKAFTD LKIVVEGREF EVHQNVLASC SLYFKDLIQR
361SVQDSGQGGR EKLELVLSNL QADVLELLLE FVYTGSLVID SANAKTLLEA ASKFQFHTFC
421KVCVSFLEKQ LTASNCLGVL AMAEAMQCSE LYHMAKAFAL QIFPEVAAQE EILSISKDDF
481IAYVSNDSLN TKAEELVYET VIKWIKKDPA TRTQYAAELL AVVRLPFIHP SYLLNVVDNE
541ELIKSSEACR DLVNEAKRYH MLPHARQEMQ TPRTRPRLSA GVAEVIVLVG GRQMVGMTQR
601SLVAVTCWNP QNNKWYPLAS LPFYDREFFS VVSAGDNIYL SGGMESGVTL ADVWCYMSLL
661DNWNLVSRMT VPRCRHNSLV YDGKIYTLGG LGVAGNVDHV ERYDTITNQW EAVAPLPKAV
721HSAAATVCGG KIYVFGGVNE AGRAAGVLQS YVPQTNTWSF IESPMIDNKY APAVTLNGFV
781FILGGAYARA TTIYDPEKGN IKAGPNMNHS RQFCSAVVLD GKIYATGGIV SSEGPALGNM
841EAYEPTTNTW TLLPHMPCPV FRHGCVVIKK YIQSG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

875 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.