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KLHL26 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL26 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111836-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHL26 (NM_018316), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Kelch-like 26 (Drosophila) | Kelch-like protein 26 | KLHL26
Origin Species
Human
NCBI GenBank
NM_018316
SwissProt
Q53HC5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68 kda
Protein Type
Lysate
Amino Acid Sequence
1MAESGGSSGG AGGGGAFGAG PGPERPNSTA DKNGALKCTF SAPSHSTSLL QGLATLRAQG
61QLLDVVLTIN REAFPAHKVV LAACSDYFRA MFTGGMREAS QDVIELKGVS ARGLRHIIDF
121AYSAEVTLDL DCVQDVLGAA VFLQMLPVVE LCEEFLKAAM SVETCLNIGQ MATTFSLASL
181RESVDAFTFR HFLQIAEEED FLRLPLERLV FFLQSNRLQS CAEIDLFRAA VRWLQHDPAR
241RPRASHVLCH IRFPLMQSSE LVDSVQTLDI MVEDVLCRQY LLEAFNYQVL PFRQHEMQSP
301RTAVRSDVPS LVTFGGTPYT DSDRSVSSKV YQLPEPGARH FRELTEMEVG CSHTCVAVLD
361NFVYVAGGQH LQYRSGEGAV DACYRYDPHL NRWLRLQAMQ ESRIQFQLNV LCGMVYATGG
421RNRAGSLASV ERYCPRRNEW GYACSLKRRT WGHAGAASGG RLYISGGYGI SVEDKKALHC
481YDPVADQWEF KAPMSEPRVL HAMVGAGGRI YALGGRMDHV DRCFDVLAVE YYVPETDQWT
541SVSPMRAGQS EAGCCLLERK IYIVGGYNWR LNNVTGIVQV YNTDTDEWER DLHFPESFAG
601IACAPVLLPR AGTRR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

615 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.