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KLHL23 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL23 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KLHL23 antibodies →
$504.00each
Catalog Number: LS-G106876-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHL23 (NM_144711), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DITHP | Kelch-like protein 23 | Kelch-like 23 (Drosophila) | KLHL23
Origin Species
Human
SwissProt
Q8NBE8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MALKGQEDYI YLFKDSTHPV DFLDAFRTFY LDGLFTDITL QCPSGIIFHC HRAVLAACSN
61YFKAMFTADM KEKFKNKIKL SGIHHDILEG LVNYAYTSQI EITKRNVQSL LEAADLLQFL
121SVKKACERFL VRHLDIDNCI GMHSFAEFHV CPELEKESRR ILCSKFKEVW QQEEFLEISL
181EKFLFILSRK NLSVWKEEAI IEPVIKWTAH DVENRIECLY NLLSYINIDI DPVYLKTALG
241LQRSCLLTEN KIRSLIYNAL NPMHKEISQR STATMYIIGG YYWHPLSEVH IWDPLTNVWI
301QGAEIPDYTR ESYGVTCLGP NIYVTGGYRT DNIEALDTVW IYNSESDEWT EGLPMLNARY
361YHCAVTLGGC VYALGGYRKG APAEEAEFYD PLKEKWIPIA NMIKGVGNAT ACVLHDVIYV
421IGGHCGYRGS CTYDKVQSYN SDINEWSLIT SSPHPEYGLC SVPFENKLYL VGGQTTITEC
481YDPEQNEWRE IAPMMERRME CGAVIMNGCI YVTGGYSYSK GTYLQSIEKY DPDLNKWEIV
541GNLPSAMRSH GCVCVYNV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

558 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.