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KLHL17 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL17 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G103781-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHL17 (NM_198317), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Actinfilin | Kelch-like 17 (Drosophila) | KLHL17 | RP11-54O7.6 | Kelch-like protein 17
Origin Species
Human
NCBI GenBank
NM_198317
SwissProt
Q6TDP4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQPRSERPAG RTQSPEHGSP GPGPEAPPPP PPQPPAPEAE RTRPRQARPA APMEGAVQLL
61SREGHSVAHN SKRHYHDAFV AMSRMRQRGL LCDIVLHVAA KEIRAHKVVL ASCSPYFHAM
121FTNEMSESRQ THVTLHDIDP QALDQLVQFA YTAEIVVGEG NVQTLLPAAS LLQLNGVRDA
181CCKFLLSQLD PSNCLGIRGF ADAHSCSDLL KAAHRYVLQH FVDVAKTEEF MLLPLKQVLE
241LVSSDSLNVP SEEEVYRAVL SWVKHDVDAR RQHVPRLMKC VRLPLLSRDF LLGHVDAESL
301VRHHPDCKDL LIEALKFHLL PEQRGVLGTS RTRPRRCEGA GPVLFAVGGG SLFAIHGDCE
361AYDTRTDRWH VVASMSTRRA RVGVAAVGNR LYAVGGYDGT SDLATVESYD PVTNTWQPEV
421SMGTRRSCLG VAALHGLLYS AGGYDGASCL NSAERYDPLT GTWTSVAAMS TRRRYVRVAT
481LDGNLYAVGG YDSSSHLATV EKYEPQVNVW SPVASMLSRR SSAGVAVLEG ALYVAGGNDG
541TSCLNSVERY SPKAGAWESV APMNIRRSTH DLVAMDGWLY AVGGNDGSSS LNSIEKYNPR
601TNKWVAASCM FTRRSSVGVA VLELLNFPPP SSPTLSVSST SL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

642 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.