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KLHL14 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL14 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110884-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHL14 (NM_020805), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1384 | KLHL14 | Kelch-like 14 (Drosophila) | Kelch-like protein 14 | Printor | Protein interactor of torsinA
Origin Species
Human
NCBI GenBank
AB037805
SwissProt
Q9P2G3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSRSGDRTST FDPSHSDNLL HGLNLLWRKQ LFCDVTLTAQ GQQFHCHKAV LASCSQYFRS
61LFSSHPPLGG GVGGQDGLGA PKDQQQPPQQ QPSQQQQPPP QEEPGTPSSS PDDKLLTSPR
121AINNLVLQGC SSIGLRLVLE YLYTANVTLS LDTVEEVLSV SKILHIPQVT KLCVQFLNDQ
181ISVQNYKQVC KIAALHGLEE TKKLANKYLV EDVLLLNFEE MRALLDSLPP PVESELALFQ
241MSVLWLEHDR ETRMQYAPDL MKRLRFALIP APELVERVQS VDFMRTDPVC QKLLLDAMNY
301HLMPFRQHCR QSLASRIRSN KKMLLLVGGL PPGPDRLPSN LVQYYDDEKK TWKILTIMPY
361NSAHHCVVEV ENFLFVLGGE DQWNPNGKHS TNFVSRYDPR FNSWIQLPPM QERRASFYAC
421RLDKHLYVIG GRNETGYLSS VECYNLETNE WRYVSSLPQP LAAHAGAVHN GKIYISGGVH
481NGEYVPWLYC YDPVMDVWAR KQDMNTKRAI HTLAVMNDRL YAIGGNHLKG FSHLDVMLVE
541CYDPKGDQWN ILQTPILEGR SGPGCAVLDD SIYLVGGYSW SMGAYKSSTI CYCPEKGTWT
601ELEGDVAEPL AGPACVTVIL PSCVPYNK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

628 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.