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KLHL11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL11 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111949-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHL11 (NM_018143), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Kelch-like 11 (Drosophila) | KLHL11 | Kelch-like protein 11
Origin Species
Human
NCBI GenBank
NM_018143
SwissProt
Q9NVR0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAAVAAAA AAAAAASLQV LEMESMETAA AGSAGLAAEV RGSGTVDFGP GPGISAMEAS
61GGDPGPEAED FECSSHCSEL SWRQNEQRRQ GLFCDITLCF GGAGGREFRA HRSVLAAATE
121YFTPLLSGQF SESRSGRVEM RKWSSEPGPE PDTVEAVIEY MYTGRIRVST GSVHEVLELA
181DRFLLIRLKE FCGEFLKKKL HLSNCVAIHS LAHMYTLSQL ALKAADMIRR NFHKVIQDEE
241FYTLPFHLIR DWLSDLEITV DSEEVLFETV LKWVQRNAEE RERYFEELFK LLRLSQMKPT
301YLTRHVKPER LVANNEVCVK LVADAVERHA LRAENIQSGT CQHPTSHVSL LPRYGQNMDV
361IMVIGGVSEG GDYLSECVGY FVDEDRWVNL PHIHNHLDGH AVAVTESYVY VAGSMEPGFA
421KTVERYNPNL NTWEHVCSLM TRKHSFGLTE VKGKLYSIGG HGNFSPGFKD VTVYNPELDK
481WHNLESAPKI LRDVKALAIE DRFVYIAART PVDRDTEDGL KAVITCYDTE TRQWQDVESL
541PLIDNYCFFQ MSVVNSNFYQ TASCCPKSYC LENEEAVRKI ASQVSDEILE SLPPEVLSIE
601GAAICYYKDD VFIIGGWKNS DDIDKQYRKE AYRYCAERKR WMLLPPMPQP RCRATACHVR
661IPYRYLHGTQ RYPMPQNLMW QKDRIRQMQE IHRHALNMRR VPSSQIEC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

708 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.