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KLHL10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHL10 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106249-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHL10 (NM_152467), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Kelch-like 10 (Drosophila) | Kelch-like protein 10 | KLHL10
Origin Species
Human
NCBI GenBank
NM_152467
SwissProt
Q6JEL2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEMESAAAST RFHQPHMERK MSAMACEIFN ELRLEGKLCD VVIKVNGFEF SAHKNILCSC
61SSYFRALFTS GWNNTEKKVY NIPGISPDMM KLIIEYAYTR TVPITPDNVE KLLAAADQFN
121IMGIVRGCCE FLKSELCLDN CIGICKFTDY YYCPELRQKA YMFILHNFEE MVKVSAEFLE
181LSVTELKDII EKDELNVKQE DAVFEAILKW ISHDPQNRKQ HISILLPKVR LALMHAEYFM
241NNVKMNDYVK DSEECKPVII NALKAMYDLN MNGPSNSDFT NPLTRPRLPY AILFAIGGWS
301GGSPTNAIEA YDARADRWVN VTCEEESPRA YHGAAYLKGY VYIIGGFDSV DYFNSVKRFD
361PVKKTWHQVA PMHSRRCYVS VTVLGNFIYA MGGFDGYVRL NTAERYEPET NQWTLIAPMH
421EQRSDASATT LYGKVYICGG FNGNECLFTA EVYNTESNQW TVIAPMRSRR SGIGVIAYGE
481HVYAVGGFDG ANRLRSAEAY SPVANTWRTI PTMFNPRSNF GIEVVDDLLF VVGGFNGFTT
541TFNVECYDEK TDEWYDAHDM SIYRSALSCC VVPGLANVEE YAARRDNFPG LALRDEVKYS
601ASTSTLPV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

608 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.