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KLHDC7A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHDC7A Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KLHDC7A antibodies →
$725.00each
Catalog Number: LS-G106346-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHDC7A (NM_152375), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_152375

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFPRGAEAQD WHLDMQLTGK VVLSAAALLL VTVAYRLYKS RPAPAQRWGG NGQAEAKEEA
61EGSGQPAVQE ASPGVLLRGP RRRRSSKRAE APQGCSCENP RGPYVLVTGA TSTDRKPQRK
121GSGEERGGQG SDSEQVPPCC PSQETRTAVG SNPDPPHFPR LGSEPKSSPA GLIAAADGSC
181AGGEPSPWQD SKPREHPGLG QLEPPHCHYV APLQGSSDMN QSWVFTRVIG VSREEAGALE
241AASDVDLTLH QQEGAPNSSY TFSSIARVRM EEHFIQKAEG VEPRLKGKVY DYYVESTSQA
301IFQGRLAPRT AALTEVPSPR PPPGSLGTGA ASGGQAGDTK GAAERAASPQ TGPWPSTRGF
361SRKESLLQIA ENPELQLQPD GFRLPAPPCP DPGALPGLGR SSREPHVQPV AGTNFFHIPL
421TPASAPQVRL DLGNCYEVLT LAKRQNLEAL KEAAYKVMSE NYLQVLRSPD IYGCLSGAER
481ELILQRRLRG RQYLVVADVC PKEDSGGLCC YDDEQDVWRP LARMPPEAVS RGCAICSLFN
541YLFVVSGCQG PGHQPSSRVF CYNPLTGIWS EVCPLNQARP HCRLVALDGH LYAIGGECLN
601SVERYDPRLD RWDFAPPLPS DTFALAHTAT VRAKEIFVTG GSLRFLLFRF SAQEQRWWAG
661PTGGSKDRTA EMVAVNGFLY RFDLNRSLGI AVYRCSASTR LWYECATYRT PYPDAFQCAV
721VDNLIYCVGR RSTLCFLADS VSPRFVPKEL RSFPAPQGTL LPTVLTLPTP DLPQTRV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

777 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.