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KLHDC4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHDC4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KLHDC4 antibodies →
$504.00each
Catalog Number: LS-G112382-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHDC4 (NM_017566), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Kelch domain containing 4 | KLHDC4
Origin Species
Human
NCBI GenBank
NM_017566
SwissProt
Q8TBB5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGKKGKKEKK GRGAEKTAAK MEKKVSKRSR KEEEDLEALI AHFQTLDAKR TQTVELPCPP
61PSPRLNASLS VHPEKDELIL FGGEYFNGQK TFLYNELYVY NTRKDTWTKV DIPSPPPRRC
121AHQAVVVPQG GGQLWVFGGE FASPNGEQFY HYKDLWVLHL ATKTWEQVKS TGGPSGRSGH
181RMVAWKRQLI LFGGFHESTR DYIYYNDVYA FNLDTFTWSK LSPSGTGPTP RSGCQMSVTP
241QGGIVVYGGY SKQRVKKDVD KGTRHSDMFL LKPEDGREDK WVWTRMNPSG VKPTPRSGFS
301VAMAPNHQTL FFGGVCDEEE EESLSGEFFN DLYFYDATRN RWFEGQLKGP KSEKKKRRRG
361RKEEPEGGSR PACGGAGTQG PVQLVKEVVA EDGTVVTIKQ VLTAPGSAGQ PRSEDEDSLE
421EAGSPAPGPC PRSNAMLAVK HGVLYVYGGM FEAGDRQVTL SDLHCLDLHR MEAWKALVEM
481DPETQEWLEE TDSEEDSEEV EGAEGGVDDE DSGEESGAED
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

520 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.