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KLHDC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLHDC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G107967-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLHDC3 (NM_057161), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ20C7.3 | Kelch domain containing 3 | KLHDC3 | HPeas | Protein Peas | PEAS | RP1-20C7.3
Origin Species
Human
SwissProt
Q9BQ90

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
42.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MLRWTVHLEG GPRRVNHAAV AVGHRVYSFG GYCSGEDYET LRQIDVHIFN AVSLRWTKLP
61PVKSAIRGQA PVVPYMRYGH STVLIDDTVL LWGGRNDTEG ACNVLYAFDV NTHKWFTPRV
121SGTVPGARDG HSACVLGKIM YIFGGYEQQA DCFSNDIHKL DTSTMTWTLI CTKGSPARWR
181DFHSATMLGS HMYVFGGRAD RFGPFHSNNE IYCNRIRVFD TRTEAWLDCP PTPVLPEGRR
241SHSAFGYNGE LYIFGGYNAR LNRHFHDLWK FNPVSFTWKK IEPKGKGPCP RRRQCCCIVG
301DKIVLFGGTS PSPEEGLGDE FDLIDHSDLH ILDFSPSLKT LCKLAVIQYN LDQSCLPHDI
361RWELNAMTTN SNISRPIVSS HG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

382 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.