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KLF17 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLF17 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105332-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLF17 (NM_173484), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Kruppel-like factor 17 | KLF17 | Novel zinc-finger protein | Zfp393 | ZNF393 | RP4-675G8.1 | Zinc finger protein 393 | Krueppel-like factor 17
Origin Species
Human
SwissProt
Q5JT82

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
42.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MYGRPQAEME QEAGELSRWQ AAHQAAQDNE NSAPILNMSS SSGSSGVHTS WNQGLPTIQH
61FPHSAEMLGS PLVSVEAPGQ NVNEGGPQFS MPLPERGMSY CPQATLTPSR MIYCQRMSPP
121QQEMTIFSGP QLMPVGEPNI PRVARPFGGN LRMPPSGLPV SASTGIPIMS HTGNPPVPYP
181GLSTVPSDET LLGPTVPSTE AQAVLPSMAQ MLPPQDAHDL GMPPAESQSL LVLGSQDSLV
241SQPDSQEGPF LPEQPGPAPQ TVEKNSRPQE GTGRRGSSEA RPYCCNYENC GKAYTKRSHL
301VSHQRKHTGE RPYSCNWESC SWSFFRSDEL RRHMRVHTRY RPYKCDQCSR EFMRSDHLKQ
361HQKTHRPGPS DPQANNNNGE QDSPPAAGP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

389 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.