Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KLF14 / BTEB5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107257-100
Catalog Number: LS-G107257
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLF14 (NM_138693), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BTEB5 | KLF14 | Krueppel-like factor 14 | Kruppel-like factor 14 | Transcription factor BTEB5 | BTE-binding protein 5
- Origin Species
- Human
- SwissProt
- Q8TD94
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 32.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSAAVACLDY FAAECLVSMS AGAVVHRRPP DPEGAGGAAG SEVGAAHPES ALPGPGPSGP61ASVPQLPQVP APSPGAGGAA PHLLAASVWA DLRGSSGEGS WENSGEAPRA SSGFSDPIPC121SVQTPCSELA PASGAAAVCA PESSSDAPAV PSAPAAPGAP AASGGFSGGA LGAGPAPAAD181QAPRRRSVTP AAKRHQCPFP GCTKAYYKSS HLKSHQRTHT GERPFSCDWL DCDKKFTRSD241ELARHYRTHT GEKRFSCPLC PKQFSRSDHL TKHARRHPTY HPDMIEYRGR RRTPRIDPPL301TSEVESSASG SGPGPAPSFT TCLSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
323 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.