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KLC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KLC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KLC2 antibodies →
$504.00each
Catalog Number: LS-G125335-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KLC2 (NM_001134774), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KLC 2 | Kinesin light chain 2 | KLC2
Origin Species
Human
NCBI GenBank
NM_022822
SwissProt
Q9H0B6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAMMVFPREE KLSQDEIVLG TKAVIQGLET LRGEHRALLA PLVAPEAGEA EPGSQERCIL
61LRRSLEAIEL GLGEAQEEKG DVPKDTLDDL FPNEDEQSPA PSPGGGDVSG QHGGYEIPAR
121LRTLHNLVIQ YASQGRYEVA VPLCKQALED LEKTSGHDHP DVATMLNILA LVYRDQNKYK
181EAAHLLNDAL AIREKTLGKD HPAVAATLNN LAVLYGKRGK YKEAEPLCKR ALEIREKVLG
241KFHPDVAKQL SNLALLCQNQ GKAEEVEYYY RRALEIYATR LGPDDPNVAK TKNNLASCYL
301KQGKYQDAET LYKEILTRAH EKEFGSVNGD NKPIWMHAEE REESKDKRRD SAPYGEYGSW
361YKACKVDSPT VNTTLRSLGA LYRRQGKLEA AHTLEDCASR NRKQGLDPAS QTKVVELLKD
421GSGRRGDRRS SRDMAGGAGP RSESDLEDVG PTAEWNGDGS GSLRRSGSFG KLRDALRRSS
481EMLVKKLQGG TPQEPPNPRM KRASSLNFLN KSVEEPTQPG GTGLSDSRTL SSSSMDLSRR
541SSLVG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

545 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.