Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIRREL2 / FILTRIN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108746-100
Catalog Number: LS-G108746
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIRREL2 (NM_032123), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FILTRIN | Kin of irregular chiasm-like 2 | Nephrin-like gene 1 | Nephrin-like protein 3 | Nephrin-like 3 | NLG1 | Kin of IRRE-like protein 2 | KIRREL2 | NEPH3
- Origin Species
- Human
- NCBI GenBank
- NM_032123
- SwissProt
- Q6UWL6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLRMRVPALL VLLFCFRGRA GPSPHFLQQP EDLVVLLGEE ARLPCALGAY WGLVQWTKSG61LALGGQRDLP GWSRYWISGN AANGQHDLHI RPVELEDEAS YECQATQAGL RSRPAQLHVL121VPPEAPQVLG GPSVSLVAGV PANLTCRSRG DARPTPELLW FRDGVLLDGA TFHQTLLKEG181TPGSVESTLT LTPFSHDDGA TFVCRARSQA LPTGRDTAIT LSLQYPPEVT LSASPHTVQE241GEKVIFLCQA TAQPPVTGYR WAKGGSPVLG ARGPRLEVVA DASFLTEPVS CEVSNAVGSA301NRSTALDVLF GPILQAKPEP VSVDVGEDAS FSCAWRGNPL PRVTWTRRGG AQVLGSGATL361RLPSVGPEDA GDYVCRAEAG LSGLRGGAAE ARLTVNAPPV VTALHSAPAF LRGPARLQCL421VFASPAPDAV VWSWDEGFLE AGSQGRFLVE TFPAPESRGG LGPGLISVLH ISGTQESDFS481RSFNCSARNR LGEGGAQASL GRRDLLPTVR IVAGVAAATT TLLMVITGVA LCCWRHSKAS541ASFSEQKNLM RIPGSSDGSS SRGPEEEETG SREDRGPIVH TDHSDLVLEE EGTLETKDPT601NGYYKVRGVS PPASPDSRVT SFQWKSPGIS NLPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
633 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.