Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIR2DS2 / CD158j Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114499-100
Catalog Number: LS-G114499
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIR2DS2 (NM_012312), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 1060P11.9.2 | 183ActI | CD158j antigen | CD158J | Killer-cell Ig-like receptor | KIR2DS2 | MHC class I NK cell receptor | NK receptor 183 ActI | p58 KIR | p58 NK receptor CL-49 | Cl-49 | NKAT-5 | NKAT5
- Origin Species
- Human
- NCBI GenBank
- NM_012312
- SwissProt
- P43631
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 33.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLMVVSMAC VGFFLLQGAW PHEGVHRKPS LLAHPGPLVK SEETVILQCW SDVRFEHFLL61HREGKYKDTL HLIGEHHDGV SKANFSIGPM MQDLAGTYRC YGSVTHSPYQ LSAPSDPLDI121VITGLYEKPS LSAQPGPTVL AGESVTLSCS SRSSYDMYHL SREGEAHERR FSAGPKVNGT181FQADFPLGPA THGGTYRCFG SFRDSPYEWS NSSDPLLVSV TGNPSNSWPS PTEPSSKTGN241PRHLHVLIGT SVXQNXPFTI LLFFLLHRWC SNKKNAAVMD QEPAGNRTVN SEDSDEQDHQ301EVSYATRTRPLEQKLISEEDLAANDILDYKDDDDKV
305 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.