Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIR2DL5A / KIR2DL5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111100-100
Catalog Number: LS-G111100
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIR2DL5A (NM_020535), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD158f1 antigen | CD158F | CD158F1 | KIR2DL5.3 | Killer-cell Ig-like receptor | KIR2DL5.1 | KIR2DL5 | KIR2DL5A
- Origin Species
- Human
- NCBI GenBank
- NM_020535
- SwissProt
- Q8N109
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLMVISMAC VGFFLLQGAW THEGGQDKPL LSAWPSAVVP RGGHVTLLCR SRLGFTIFSL61YKEDGVPVPE LYNKIFWKSI LMGPVTPAHA GTYRCRGSHP RSPIEWSAPS NPLVIVVTGL121FGKPSLSAQP GPTVRTGENV TLSCSSRSSF DMYHLSREGR AHEPRLPAVP SVNGTFQADF181PLGPATHGGT YTCFGSLHDS PYEWSDPSDP LLVSVTGNSS SSSSSPTEPS SKTGIRRHLH241ILIGTSVAII LFIILFFFLL HCCCSNKKNA AVMDQEPAGD RTVNREDSDD QDPQEVTYAQ301LDHCVFTQTK ITSPSQRPKT PPTDTTMYME LPNAKPRSLS PAHKHHSQAL RGSSRETTAL361SQNRVASSHV PAAGITRTRPLEQKLISEEDLAANDILDYKDDDDKV
375 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.