Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIR2DL2 / CD158b Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114099-100
Catalog Number: LS-G114099
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIR2DL2 (NM_014219), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD158B1 | Cl-43 | CD158k | MHC class I NK cell receptor | NKAT6 | NKAT-6 | p58 NK receptor CL-43 | p58.2 | CD158b | CD158b1 antigen | KIR2DL2
- Origin Species
- Human
- NCBI GenBank
- NM_014219
- SwissProt
- P43627
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.47 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLMVVSMAC VGFFLLQGAW PHEGVHRKPS LLAHPGRLVK SEETVILQCW SDVRFEHFLL61HREGKFKDTL HLIGEHHDGV SKANFSIGPM MQDLAGTYRC YGSVTHSPYQ LSAPSDPLDI121VITGLYEKPS LSAQPGPTVL AGESVTLSCS SRSSYDMYHL SREGEAHECR FSAGPKVNGT181FQADFPLGPA THGGTYRCFG SFRDSPYEWS NSSDPLLVSV IGNPSNSWPS PTEPSSKTGN241PRHLHILIGT SVVIILFILL FFLLHRWCSN KKNAAVMDQE SAGNRTANSE DSDEQDPQEV301TYTQLNHCVF TQRKITRPSQ RPKTPPTDII VYAELPNAES RSKVVSCPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
348 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.