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KIFC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIFC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G125007-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIFC3 (NM_001130100), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIFC3 | Kinesin family member C3 | Kinesin-like protein KIFC3
Origin Species
Human
NCBI GenBank
NM_005550
SwissProt
Q9BVG8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVPSRRTWNL GATPSLRGLW RVGRAPEPEP GMARPAPAPA SPAARPFPHT GPGRLRTGRG
61KDTPVCGDED SSARSAARPA LAQCRALSVD WAGPGSPHGL YLTLQVEHLK EKLISQAQEV
121SRLRSELGGT DLEKHRDLLM VENERLRQEM RRCEAELQEL RTKPAGPCPG CEHSQESAQL
181RDKLSQLQLE MAESKGMLSE LNLEVQQKTD RLAEVELRLK DCLAEKAQEE ERLSRRLRDS
241HETIASLRAQ SPPVKYVIKT VEVESSKTKQ ALSESQARNQ HLQEQVAMQR QVLKEMEQQL
301QSSHQLTARL RAQIAMYESE LERAHGQMLE EMQSLEEDKN RAIEEAFARA QVEMKAVHEN
361LAGVRTNLLT LQPALRTLTN DYNGLKRQVR GFPLLLQEAL RSVKAEIGQA IEEVNSNNQE
421LLRKYRRELQ LRKKCHNELV RLKGNIRVIA RVRPVTKEDG EGPEATNAVT FDADDDSIIH
481LLHKGKPVSF ELDKVFSPQA SQQDVFQEVQ ALVTSCIDGF NVCIFAYGQT GAGKTYTMEG
541TAENPGINQR ALQLLFSEVQ EKASDWEYTI TVSAAEIYNE VLRDLLGKEP QEKLEIRLCP
601DGSGQLYVPG LTEFQVQSVD DINKVFEFGH TNRTTEFTNL NEHSSRSHAL LIVTVRGVDC
661STGLRTTGKL NLVDLAGSER VGKSGAEGSR LREAQHINKS LSALGDVIAA LRSRQGHVPF
721RNSKLTYLLQ DSLSGDSKTL MVVQVSPVEK NTSETLYSLK FAERVRSVEL GPGLRRAELG
781SWSSQEHLEW EPACQTPQPS ARAHSAPSSG TSSRPGSIRR KLQPSA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

826 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.