Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIFC2 / Kinesin C2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106592-100
Catalog Number: LS-G106592
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIFC2 (NM_145754), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Kinesin C2 | KIFC2 | Kinesin family member C2 | Kinesin-like protein KIFC2
- Origin Species
- Human
- NCBI GenBank
- NM_145754
- SwissProt
- Q96AC6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 90 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MYAFYSLLIY IFYSLFRRDG GAAAAAEPGD PAQRARKPRG RRRPDLPAPE LWTELTGLAA61SSEPEDGSEG AAEGRAAAVS LEEALLRLAE FLSVQLGAEE SCGGPADLGQ SGEVPSLLTV121TSQLLALLAW LRSPRGRQAL LQGTQPAPRV RPPSPDGSTS QEESPSHFTA VPGEPLGDET181QGQQPLQLEE DQRAWQRLEQ LILGQLEELK QQLEQQEEEL GRLRLGVGAT DSEKRVQHLT241LENEALKQSL SLMRDLLLHW GPGPPIRAPQ EEAEALLELQ GRLQEAQDTT EALRAQLGVQ301EVQLQGLQGA LQQLQQETEQ NCRRELQQMH GQLAGLRARM ASLRQGCGDL RGLVSTFTQS361CQGSLSEARG QVSWALGALS SGGPGTQLPE GQQGPPAGCP GRLPELKGNI RVLCRLRPGT421SSSLVSVEPG PGGTVTTCYR GRHRRFRLDW VFPPDASQEE VFRELEPAVL SCLRGYSVCI481FTYGQTGTGK TYSMEGPPED PGIVPRALQS LFREMGAGRQ HRVTLSMVEI YNEAVRDLLA541PGPPERLAVR QGPEGQGGIQ VAGLTHWDVP NLETLHQMLK LGRSNRATAA TAMNQRSSRS601HALVTLTLRA ASPPRAPGTA GTLHLVDLAG SERARKAGAA GPPRGDPDGA RRLREAQTIN661RSLLALGGVM AALRAHRPHV PFRDSQLTRL LQPALGPGTT AVLLLQVGAG AGQVCACRSP721PTRARPPAPL ARRSPRGRRI SGRQSAPSSS PTEWVKWSWG QPGAAGSRAP PGRLLPSAPT781LRSPGPPAPL RRPLAVLHAP VPTTARARLS RPQRACPSSP GSRPCPWGLR PGLCWQRRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
838 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.