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KIF9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIF9 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIF9 antibodies →
$504.00each
Catalog Number: LS-G104158-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIF9 (NM_182902), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Kinesin family member 9 | Kinesin-like protein KIF9 | KIF9 | Kinesin protein 9
Origin Species
Human
NCBI GenBank
NM_001134878
SwissProt
Q9HAQ2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MGTRKKVHAF VRVKPTDDFA HEMIRYGDDK RSIDIHLKKD IRRGVVNNQQ TDWSFKLDGV
61LHDASQDLVY ETVAKDVVSQ ALDGYNGTIM CYGQTGAGKT YTMMGATENY KHRGILPRAL
121QQVFRMIEER PTHAITVRVS YLEIYNESLF DLLSTLPYVG PSVTPMTIVE NPQGVFIKGL
181SVHLTSQEED AFSLLFEGET NRIIASHTMN KNSSRSHCIF TIYLEAHSRT LSEEKYITSK
241INLVDLAGSE RLGKSGSEGQ VLKEATYINK SLSFLEQAII ALGDQKRDHI PFRQCKLTHA
301LKDSLGGNCN MVLVTNIYGE AAQLEETLSS LRFASRMKLV TTEPAINEKY DAERMVKNLE
361KELALLKQEL AIHDSLTNRT FVTYDPMDEI QIAEINSQVR RYLEGTLDEI DIISLRQIKE
421VFNQFRVVLS QQEQEVESTL RRKYTLIDRN DFAAISAIQK AGLVDVDGHL VGEPEGQNFG
481LGVAPFSTKP GKKAKSKKTF KEPLSSLARK EGASSPVNGK DLDYVSTSKT QLVPSSKDGD
541VKDMLSRDRE TSSIEPLPSD SPKEELRPIR PDTPPSKPVA FEEFKNEQGS EINRIFKENK
601SILNERRKRA SETTQHINAI KREIDVTKEA LNFQKSLWEK QGKYENKGLM IIDEEEFLLI
661LKLKDLKKQY RSEYQDLRDL RAEIQYCQHL VDQCRHRLLM EFDIWYNESF VIPEDMQMAL
721KPGGSIRPGM VPVNRIVSLG EDDQDKFSQL QQRVLPEGPD SISFYNAKVK IEQKHNYLKT
781MMGLQQAHRK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

790 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.