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KIF7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIF7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G102568-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIF7 (NM_198525), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ACLS | EQYK340 | HLS2 | KIF7 | Kinesin family member 7 | JBTS12 | Kinesin-like protein KIF7 | UNQ340
Origin Species
Human
SwissProt
Q2M1P5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEQYKLQSDR LREQQEEMVE LRLRLELVRP GWGGLRLLNG LPPGSFVPRP HTAPLGGAHA
61HVLGMVPPAC LPGDEVGSEQ RGEQVTNGRE AGAELLTEVN RLGSGSSAAS EEEEEEEEPP
121RRTLHLRRNR ISNCSQRAGA RPGSLPERKG PELCLEELDA AIPGSRAVGG SKARVQARQV
181PPATASEWRL AQAQQKIREL AINIRMKEEL IGELVRTGKA AQALNRQHSQ RIRELEQEAE
241QVRAELSEGQ RQLRELEGKE LQDAGERSRL QEFRRRVAAA QSQVQVLKEK KQATERLVSL
301SAQSEKRLQE LERNVQLMRQ QQGQLQRRLR EETEQKRRLE AEMSKRQHRV KELELKHEQQ
361QKILKIKTEE IAAFQRKRRS GSNGSVVSLE QQQKIEEQKK WLDQEMEKVL QQRRALEELG
421EELHKREAIL AKKEALMQEK TGLESKRLRS SQALNEDIVR VSSRLEHLEK ELSEKSGQLR
481QGSAQSQQQI RGEIDSLRQE KDSLLKQRLE IDGKLRQGSL LSPEEERTLF QLDEAIEALD
541AAIEYKNEAI TCRQRVLRAS ASLLSQCEMN LMAKLSYLSS SETRALLCKY FDKVVTLREE
601QHQQQIAFSE LEMQLEEQQR LVYWLEVALE RQRLEMDRQL TLQQKEHEQN MQLLLQQSRD
661HLGEGLADSR RQYEARIQAL EKELGRYMWI NQELKQKLGG VNAVGHSRGG EKRSLCSEGR
721QAPGNEDELH LAPELLWLSP LTEGAPRTRE ETRDLVHAPL PLTWKRSSLC GEEQGSPEEL
781RQREAAEPLV GRVLPVGEAG LPWNFGPLSK PRRELRRASP GMIDVRKNPL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

830 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.