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KIF6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIF6 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106802-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIF6 (NM_145027), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C6orf102 | DJ188D3.1 | DJ1043E3.1 | DJ137F1.4 | Kinesin family member 6 | Kinesin-like protein KIF6 | KIF6
Origin Species
Human
NCBI GenBank
NM_145027
SwissProt
Q6ZMV9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVKQTIQIFA RVKPPVRKHQ QGIYSIDEDE KLIPSLEIIL PRDLADGFVN NKRESYKFKF
61QRIFDQDANQ ETVFENIAKP VAGSVLAGYN GTIFAYGQTG SGKTFTITGG AERYSDRGII
121PRTLSYIFEQ LQKDSSKIYT THISYLEIYN ECGYDLLDPR HEASSLEDLP KVTILEDPDQ
181NIHLKNLTLH QATTEEEALN LLFLGDTNRM IAETPMNQAS TRSHCIFTIH LSSKEPGSAT
241VRHAKLHLVD LAGSERVAKT GVGGHLLTEA KYINLSLHYL EQVIIALSEK HRSHIPYRNS
301MMTSVLRDSL GGNCMTTMIA TLSLEKRNLD ESISTCRFAQ RVALIKNEAV LNEEINPRLV
361IKRLQKEIQE LKDELAMVTG EQRTEALTEA ELLQLEKLIT SFLEDQDSDS RLEVGADMRK
421VHHCFHHLKK LLNDKKILEN NTVSSESKDQ DCQEPLKEEE YRKLRDILKQ RDNEINILVN
481MLKKEKKKAQ EALHLAGMDR REFRQSQSPP FRLGNPEEGQ RMRLSSAPSQ AQDFSILGKR
541SSLLHKKIGM REEMSLGCQE AFEIFKRDHA DSVTIDDNKQ ILKQRFSEAK ALGESINEAR
601SKIGHLKEEI TQRHIQQVAL GISENMAVPL MPDQQEEKLR SQLEEEKRRY KTMFTRLKAL
661KVEIEHLQLL MDKAKVKLQK EFEVWWAEEA TNLQVNSPAV NSLDHTKPFL QTSDSQHEWS
721QLLSNKSSGG WEVQDQGTGR FDVCDVNARK ILPSPCPSPH SQKQSSTSTP LEDSIPKRPV
781SSIPLTGDSQ TDSDIIAFIK ARQSILQKQC LGSN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

814 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.