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KIF4B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIF4B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIF4B antibodies →
$725.00each
Catalog Number: LS-G119060-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIF4B (NM_001099293), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Kinesin family member 4B | Chromokinesin-B | KIF4B
Origin Species
Human
NCBI GenBank
AF241316

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
139.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKEEVKGIPV RVALRCRPLV PKEISEGCQM CLSFVPGETQ VVVGTDKSFT YDFVFDPCTE
61QEEVFNKAVA PLIKGIFKGY NATVLAYGQT GSGKTYSMGG AYTAEQENEP TVGIIPRVIQ
121LLFKEIDKKS DFEFTLKVSY LEIYNEEILD LLCPSREKAQ INIREDPKEG IKIVGLTEKT
181VLVALDTVSC LEQGNNSRTV ASTAMNSQSS RSHAIFTISI EQRKKSDKNC SFRSKLHLVD
241LAGSERQKKT KAEGDRLKEG ININRGLLCL GNVISALGDD KKGSFVPYRD SKLTRLLQDS
301LGGNSHTLMI ACVSPADSNL EETLSTLRYA DRARKIKNKP IVNIDPHTAE LNHLKQQVQQ
361LQVLLLQAHG GTLPGSINAE PSENLQSLME KNQSLVEENE KLSRCLSKAA GQTAQMLERI
421ILTEQVNEKL NAKLEELRQH VACKLDLQKL VETLEDQELK ENVEIICNLQ QLITQLSDET
481VACTAAAIDT AVEEEAQVET SPETSRSSDA FTTQHALHQA QMSKEVVELN NALALKEALV
541RKMTQNDNQL QPIQFQYQDN IKNLELEVIN LQKEKEELVR ELQTAKKNVN QAKLSEHRHK
601LLQELEGQIA DLKKKLNEQS KLLKLKESTE RTVSKLNQEI WMMKNQRVQL MRQMKEDAEK
661FRQWKQKKDK EVIQLKERDR KRQYELLKLE RNFQKQSSVL RRKTEEAAAA NKRLKDALQK
721QREVTDKRKE TQSHGKEGIA ARVRNWLGNE IEVMVSTEEA KRHLNDLLED RKILAQDVVQ
781LKEKKESREN PPPKLRKCTF SLSEVHGQVL ESEDCITKQI ESLETEMELR SAQIADLQQK
841LLDAESEDRP KQCWENIATI LEAKCALKYL IGELVSSKIH VTKLENSLRQ SKASCADMQK
901MLFEEQNHFS EIETELQAEL VRMEQQHQEK VLYLVSQLQE SQMAEKQLEK SASEKEQQLV
961STLQCQDEEL EKMREVCEQN QQLLQENEII KQKLILLQVA SRQKHLPNDT LLSPDSSFEY
1021IPPKPKPSRV KEKFLEQSMD IEDLKYCSEH SVNEHEDGDG DGDSDEGDDE EWKPTKLVKV
1081SRKNIQGCSC KGWCGNKQCG CRKQKSDCGV DCSCDPTKCR NRQQGKDSLG TVEQTQDSEG
1141SFKLEDPTEV TPGLSFFNPV CATPNSKILK EMCDMEQVLS KKTAPAPSPF DLPESKHGAT
1201EYQQNKPPGK KKKRALASNT SFFSGCSPIE EEAH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1234 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.