LSBio
KIF3C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIF3C Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIF3C antibodies →
$725.00each
Catalog Number: LS-G118074-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIF3C (NM_002254), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIF3C variant protein | KIF3C | Kinesin family member 3C | Kinesin-like protein KIF3C
Origin Species
Human
NCBI GenBank
NM_002254
SwissProt
O14782

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASKTKASEA LKVVARCRPL SRKEEAAGHE QILTMDVKLG QVTLRNPRAA PGELPKTFTF
61DAVYDASSKQ ADLYDETVRP LIDSVLQGFN GTVFAYGQTG TGKTYTMQGT WVEPELRGVI
121PNAFEHIFTH ISRSQNQQYL VRASYLEIYQ EEIRDLLSKE PGKRLELKEN PETGVYIKDL
181SSFVTKNVKE IEHVMNLGNQ TRAVGSTHMN EVSSRSHAIF IITVECSERG SDGQDHIRVG
241KLNLVDLAGS ERQNKAGPNT AGGAATPSSG GGGGGGGSGG GAGGERPKEA SKINLSLSAL
301GNVIAALAGN RSTHIPYRDS KLTRLLQDSL GGNAKTIMVA TLGPASHSYD ESLSTLRFAN
361RAKNIKNKPR VNEDPKDTLL REFQEEIARL KAQLEKRGML GKRPRRKSSR RKKAVSAPPG
421YPEGPVIEAW VAEEEDDNNN NHRPPQPILE SALEKNMENY LQEQKERLEE EKAAIQDDRS
481LVSEEKQKLL EEKEKMLEDL RREQQATELL AAKYKAMESK LLIGGRNIMD HTNEQQKMLE
541LKRQEIAEQK RREREMQQEM MLRDEETMEL RGTYTSLQQE VEVKTKKLKK LYAKLQAVKA
601EIQDQHDEYI RVRQDLEEAQ NEQTRELKLK YLIIENFIPP EEKNKIMNRL FLDCEEEQWK
661FQPLVPAGVS SSQMKKRPTS AVGYKRPISQ YARVAMAMGS HPRYRAENIM FLELDVSPPA
721VFEMEFSHDQ EQDPRALHME RLMRLDSFLE RPSTSKVRKS RSWCQSPQRP PPSTTHASLA
781SASLRPATVA DHE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

793 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.