Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIF2C / MCAK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100930-100
Catalog Number: LS-G100930
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MCAK(KIF2C) (NM_006845), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MCAK | KIF2C | Kinesin family member 2C | Kinesin-like protein 6 | Kinesin-like protein KIF2C | KNSL6 | Kinesin-like 6
- Origin Species
- Human
- NCBI GenBank
- NM_006845
- SwissProt
- Q99661
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 81.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAMDSSLQAR LFPGLAIKIQ RSNGLIHSAN VRTVNLEKSC VSVEWAEGGA TKGKEIDFDD61VAAINPELLQ LLPLHPKDNL PLQENVTIQK QKRRSVNSKI PAPKESLRSR STRMSTVSEL121RITAQENDME VELPAAANSR KQFSVPPAPT RPSCPAVAEI PLRMVSEEME EQVHSIRGSS181SANPVNSVRR KSCLVKEVEK MKNKREEKKA QNSEMRMKRA QEYDSSFPNW EFARMIKEFR241ATLECHPLTM TDPIEEHRIC VCVRKRPLNK QELAKKEIDV ISIPSKCLLL VHEPKLKVDL301TKYLENQAFC FDFAFDETAS NEVVYRFTAR PLVQTIFEGG KATCFAYGQT GSGKTHTMGG361DLSGKAQNAS KGIYAMASRD VFLLKNQPCY RKLGLEVYVT FFEIYNGKLF DLLNKKAKLR421VLEDGKQQVQ VVGLQEHLVN SADDVIKMID MGSACRTSGQ TFANSNSSRS HACFQIILRA481KGRMHGKFSL VDLAGNERGA DTSSADRQTR MEGAEINKSL LALKECIRAL GQNKAHTPFR541ESKLTQVLRD SFIGENSRTC MIATISPGIS SCEYTLNTLR YADRVKELSP HSGPSGEQLI601QMETEEMEAC SNGALIPGNL SKEEEELSSQ MSSFNEAMTQ IRELEEKAME ELKEIIQQGP661DWLELSEMTE QPDYDLETFV NKAESALAQQ AKHFSALPDV IKALRLAMQL EEQASRQISS721KKRPQSGPTR TRRLEQKLIS EEDLAANDIL DYKDDDDKV
759 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.