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KIF2B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIF2B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G108742-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIF2B (NM_032559), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIF2B | Kinesin family member 2B | Kinesin-like protein KIF2B | Kinesin protein
Origin Species
Human
NCBI GenBank
NM_032559
SwissProt
Q8N4N8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MASQFCLPES PCLSPLKPLK PHFGDIQEGI YVAIQRSDKR IHLAVVTEIN RENYWVTVEW
61VEKAVKKGKK IDLETILLLN PALDSAEHPM PPPPLSPLAL APSSAIRDQR TATKWVAMIP
121QKNQTASGDS LDVRVPSKPC LMKQKKSPCL WEIQKLQEQR EKRRRLQQEI RARRALDVNT
181RNPNYEIMHM IEEYRRHLDS SKISVLEPPQ EHRICVCVRK RPLNQRETTL KDLDIITVPS
241DNVVMVHESK QKVDLTRYLQ NQTFCFDHAF DDKASNELVY QFTAQPLVES IFRKGMATCF
301AYGQTGSGKT YTMGGDFSGT AQDCSKGIYA LVAQDVFLLL RNSTYEKLDL KVYGTFFEIY
361GGKVYDLLNW KKKLQVLEDG NQQIQVVGLQ EKEVCCVEEV LNLVEIGNSC RTSRQTSVNA
421HSSRSHAVFQ IILKSGGIMH GKFSLVDLAG NERGADTTKA SRKRQLEGAE INKSLLALKE
481CILALGQNKP HTPFRASKLT LVLRDSFIGQ NSSTCMIATI SPGMTSCENT LNTLRYANRV
541KKLNVDVRPY HRGHYPIGHE APRMLKSHIG NSEMSLQRDE FIKIPYVQSE EQKEIEEVET
601LPTLLGKDTT ISGKGSSQWL ENIQERAGGV HHDIDFCIAR SLSILEQKID ALTEIQKKLK
661LLLADLHVKS KVE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

673 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.