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KIF2A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIF2A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G116597-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Kinesin Heavy Chain 2(KIF2A) (NM_004520), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KNS2 | KIF2 | Kinesin heavy chain member 2A | KIF2A | Kinesin heavy chain member 2 | Kinesin | heavy chain | 2 | Kinesin-like protein KIF2A | Kinesin-2
Origin Species
Human
NCBI GenBank
NM_004520
SwissProt
O00139

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.96 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVTSLNEDNE SVTVEWIENG DTKGKEIDLE SIFSLNPDLV PDEEIEPSPE TPPPPASSAK
61VNKIVKNRRT VASIKNDPPS RDNRVVGSAR ARPSQFPEQS SSAQQNGSVS DISPVQAAKK
121EFGPPSRRKS NCVKEVEKLQ EKREKRRLQQ QELREKRAQD VDATNPNYEI MCMIRDFRGS
181LDYRPLTTAD PIDEHRICVC VRKRPLNKKE TQMKDLDVIT IPSKDVVMVH EPKQKVDLTR
241YLENQTFRFD YAFDDSAPNE MVYRFTARPL VETIFERGMA TCFAYGQTGS GKTHTMGGDF
301SGKNQDCSKG IYALAARDVF LMLKKPNYKK LELQVYATFF EIYSGKVFDL LNRKTKLRVL
361EDGKQQVQVV GLQEREVKCV EDVLKLIDIG NSCRTSGQTS ANAHSSRSHA VFQIILRRKG
421KLHGKFSLID LAGNERGADT SSADRQTRLE GAEINKSLLA LKECIRALGR NKPHTPFRAS
481KLTQVLRDSF IGENSRTCMI ATISPGMASC ENTLNTLRYA NRVKELTVDP TAAGDVRPIM
541HHPPNQIDDL ETQWGVGSSP QRDDLKLLCE QNEEEVSPQL FTFHEAVSQM VEMEEQVVED
601HRAVFQESIR WLEDEKALLE MTEEVDYDVD SYATQLEAIL EQKIDILTEL RDKVKSFRAA
661LQEEEQASKQ INPKRPRAL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

679 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.