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KIF24 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIF24 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIF24 antibodies →
$725.00each
Catalog Number: LS-G103928-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIF24 (NM_194313), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA571F15.4 | KIF24 | Kinesin family member 24 | Kinesin-like protein KIF24 | C9orf48
Origin Species
Human
NCBI GenBank
AK001795

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
151.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASWLYECLC EAELAQYYSH FTALGLQKID ELAKITMKDY SKLGVHDMND RKRLFQLIKI
61IKIMQEEDKA VSIPERHLQT SSLRIKSQEL RSGPRRQLNF DSPADNKDRN ASNDGFEMCS
121LSDFSANEQK STYLKVLEHM LPDDSQYHTK TGILNATAGD SYVQTEISTS LFSPNYLSAI
181LGDCDIPIIQ RISHVSGYNY GIPHSCIRQN TSEKQNPWTE MEKIRVCVRK RPLGMREVRR
241GEINIITVED KETLLVHEKK EAVDLTQYIL QHVFYFDEVF GEACTNQDVY MKTTHPLIQH
301IFNGGNATCF AYGQTGAGKT YTMIGTHENP GLYALAAKDI FRQLEVSQPR KHLFVWISFY
361EIYCGQLYDL LNRRKRLFAR EDSKHMVQIV GLQELQVDSV ELLLEVILKG SKERSTGATG
421VNADSSRSHA VIQIQIKDSA KRTFGRISFI DLAGSERAAD ARDSDRQTKM EGAEINQSLL
481ALKECIRALD QEHTHTPFRQ SKLTQVLKDS FIGNAKTCMI ANISPSHVAT EHTLNTLRYA
541DRVKELKKGI KCCTSVTSRN RTSGNSSPKR IQSSPGALSE DKCSPKKVKL GFQQSLTVAA
601PGSTRGKVHP LTSHPPNIPF TSAPKVSGKR GGSRGSPSQE WVIHASPVKG TVRSGHVAKK
661KPEESAPLCS EKNRMGNKTV LGWESRASGP GEGLVRGKLS TKCKKVQTVQ PVQKQLVSRV
721ELSFGNAHHR AEYSQDSQRG TPARPASEAW TNIPPHQKER EEHLRFYHQQ FQQPPLLQQK
781LKYQPLKRSL RQYRPPEGQL TNETPPLFHS YSENHDGAQV EELDDSDFSE DSFSHISSQR
841ATKQRNTLEN SEDSFFLHQT WGQGPEKQVA ERQQSLFSSP RTGDKKDLTK SWVDSRDPIN
901HRRAALDHSC SPSKGPVDWS RENSTSSGPS PRDSLAEKPY CSQVDFIYRQ ERGGGSSFDL
961RKDASQSEVS GENEGNLPSP EEDGFTISLS HVAVPGSPDQ RDTVTTPLRE VSADGPIQVT
1021STVKNGHAVP GEDPRGQLGT HAEYASGLMS PLTMSLLENP DNEGSPPSEQ LVQDGATHSL
1081VAESTGGPVV SHTVPSGDQE AALPVSSATR HLWLSSSPPD NKPGGDLPAL SPSPIRQHPA
1141DKLPSREADL GEACQSRETV LFSHEHMGSE QYDADAEETG LDGSWGFPGK PFTTIHMGVP
1201HSGPTLTPRT GSSDVADQLW AQERKHPTRL GWQEFGLSTD PIKLPCNSEN VTWLKPRPIS
1261RCLARPSSPL VPSCSPKTAG TLRQPTLEQA QQVVIRAHQE QLDEMAELGF KEETLMSQLA
1321SNDFEDFVTQ LDEIMVLKSK CIQSLRSQLQ LYLTCHGPTA APEGTVPS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1368 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.