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KIF21B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIF21B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIF21B antibodies →
$725.00each
Catalog Number: LS-G112360-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIF21B (NM_017596), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP434J212 | KIAA0449 | Kinesin-like protein KIF21B | KIF21B | Kinesin family member 21B
Origin Species
Human
NCBI GenBank
AL137395

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
181.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGQGDCCVK VAVRIRPQLS KEKIEGCHIC TSVTPGEPQV LLGKDKAFTY DFVFDLDTWQ
61EQIYSTCVSK LIEGCFEGYN ATVLAYGQTG AGKTYTMGTG FDMATSEEEQ GIIPRAIAHL
121FGGIAERKRR AQEQGVAGPE FKVSAQFLEL YNEEILDLFD STRDPDTRHR RSNIKIHEDA
181NGGIYTTGVT SRLIHSQEEL IQCLKQGALS RTTASTQMNV QSSRSHAIFT IHLCQMRMCT
241QPDLVNEAVT GLPDGTPPSS EYETLTAKFH FVDLAGSERL KRTGATGERA KEGISINCGL
301LALGNVISAL GDQSKKVVHV PYRDSKLTRL LQDSLGGNSQ TIMIACVSPS DRDFMETLNT
361LKYANRARNI KNKVVVNQDK TSQQISALRA EIARLQMELM EYKAGKRVIG EDGAEGYSDL
421FRENAMLQKE NGALRLRVKA MQEAIDAINN RVTQLMSQEA NLLLAKAGDG NEAIGALIQN
481YIREIEELRT KLLESEAMNE SLRRSLSRAS ARSPYSLGAS PAAPAFGGSP ASSMEDASEV
541IRRAKQDLER LKKKEVRQRR KSPEKEAFKK RAKLQQENSE ETDENEAEEE EEERDESGCE
601EEEGREDEDE DSGSEESLVD SDSDPEEKEV NFQADLADLT CEIEIKQKLI DELENSQRRL
661QTLKHQYEEK LILLQNKIRD TQLERDRVLQ NLSTMECYTE EKANKIKADY EKRLREMNRD
721LQKLQAAQKE HARLLKNQSR YERELKKLQA EVAEMKKAKV ALMKQMREEQ QRRRLVETKR
781NREIAQLKKE QRRQEFQIRA LESQKRQQEM VLRRKTQEVS ALRRLAKPMS ERVAGRAGLK
841PPMLDSGAEV SASTTSSEAE SGARSVSSIV RQWNRKINHF LGDHPAPTVN GTRPARKKFQ
901KKGASQSFSK AARLKWQSLE RRIIDIVMQR MTIVNLEADM ERLIKKREEL FLLQEALRRK
961RERLQAESPE EEKGLQELAE EIEVLAANID YINDGITDCQ ATIVQLEETK EELDSTDTSV
1021VISSCSLAEA RLLLDNFLKA SIDKGLQVAQ KEAQIRLLEG RLRQTDMAGS SQNHLLLDAL
1081REKAEAHPEL QALIYNVQQE NGYASTDEEI SEFSEGSFSQ SFTMKGSTSH DDFKFKSEPK
1141LSAQMKAVSA ECLGPPLDIS TKNITKSLAS LVEIKEDGVG FSVRDPYYRD RVSRTVSLPT
1201RGSTFPRQSR ATETSPLTRR KSYDRGQPIR STDVGFTPPS SPPTRPRNDR NVFSRLTSNQ
1261SQGSALDKGI ISPVGGAKGA RTAPLQCVSM AEGHTKPILC LDATDELLFT GSKDRSCKMW
1321NLVTGQEIAA LKGHPNNVVS IKYCSHSGLV FSVSTSYIKV WDIRDSAKCI RTLTSSGQVI
1381SGDACAATST RAITSAQGEH QINQIALSPS GTMLYAASGN AVRIWELSRF QPVGKLTGHI
1441GPVMCLTVTQ TASQHDLVVT GSKDHYVKMF ELGECVTGTI GPTHNFEPPH YDGIECLAIQ
1501GDILFSGSRD NGIKKWDLDQ QELIQQIPNA HKDWVCALAF IPGRPMLLSA CRAGVIKVWN
1561VDNFTPIGEI KGHDSPINAI CTNAKHIFTA SSDCRVKLWN YVPGLTPCLP RRVLAIKGRA
1621TTLP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1624 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.