LSBio
KIF19 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIF19 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIF19 antibodies →
$725.00each
Catalog Number: LS-G105868-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIF19 (NM_153209), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIF19 | KIF19A | Kinesin family member 19 | Kinesin-like protein KIF19
Origin Species
Human
SwissProt
Q2TAC6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
111.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKDSGDSKDQ QLMVALRVRP ISVAELEEGA TLIAHKVDEQ MVVLMDPMED PDDILRAHRS
61REKSYLFDVA FDFTATQEMV YQATTKSLIE GVISGYNATV FAYGPTGCGK TYTMLGTDQE
121PGIYVQTLND LFRAIEETSN DMEYEVSMSY LEIYNEMIRD LLNPSLGYLE LREDSKGVIQ
181VAGITEVSTI NAKEIMQLLM KGNRQRTQEP TAANQTSSRS HAVLQVTVRQ RSRVKNILQE
241VRQGRLFMID LAGSERASQT QNRGQRMKEG AHINRSLLAL GNCINALSDK GSNKYINYRD
301SKLTRLLKDS LGGNSRTVMI AHISPASSAF EESRNTLTYA GRAKNIKTRV KQNLLNVSYH
361IAQYTSIIAD LRGEIQRLKR KIDEQTGRGQ ARGRQDRGDI RHIQAEVQLH SGQGEKAGMG
421QLREQLASAF QEQMDVRRRL LELENRAMEV QIDTSRHLLT IAGWKHEKSR RALKWREEQR
481KECYAKDDSE KDSDTGDDQP DILEPPEVAA ARESIAALVD EQKQLRKQKL ALEQRCRELR
541ARGRRLEETL PRRIGSEEQR EVLSLLCRVH ELEVENTEMQ SHALLRDGAL RHRHEAVRRL
601EQHRSLCDEI IQGQRQIIDD YNLAVPQRLE ELYEVYLREL EEGSLEQATI MDQVASRALQ
661DSSLPKITPA GTSLTPDSDL ESVKTLSSDA QHLQNSALPP LSTESEGHHV FKAGTGAWQA
721KSSSVPTPPP IQLGSLVTQE APAQDSLGSW INSSPDSSEN LSEIPLSHKE RKEILTGTKC
781IWVKAARRRS RALGTEGRHL LAPATERSSL SLHSLSEGDD ARPPGPLACK RPPSPTLQHA
841ASEDNLSSST GEAPSRAVGH HGDGPRPWLR GQKKSLGKKR EESLEAKRRK RRSRSFEVTG
901QGLSHPKTHL LGPHQAERIS DHRMPVCRHP APGIRHLGKV TLPLAKVKLP PSQNTGPGDS
961SPLAVPPNPG GGSRRATRGP RLPHGTSTHG KDGCSRHN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

998 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.