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KIF18B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIF18B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIF18B antibodies →
$725.00each
Catalog Number: LS-G120229-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIF18B (NM_001080443), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVEDSTLQV VVRVRPPTPR ELDSQRRPVV QVVDERVLVF NPEEPDGGFP GLKWGGTHDG
61PKKKGKDLTF VFDRVFGEAA TQQDVFQHTT HSVLDSFLQG YNCSVFAYGA TGAGKTHTML
121GREGDPGIMY LTTVELYRRL EARQQEKHFE VLISYQEVYN EQIHDLLEPK GPLAIREDPD
181KGVVVQGLSF HQPASAEQLL EILTRGNRNR TQHPTDANAT SSRSHAIFQI FVKQQDRVPG
241LTQAVQVAKM SLIDLAGSER ASSTHAKGER LREGANINRS LLALINVLNA LADAKGRKTH
301VPYRDSKLTR LLKDSLGGNC RTVMIAAISP SSLTYEDTYN TLKYADRAKE IRLSLKSNVT
361SLDCHISQYA TICQQLQAEV AALRKKLQVY EGGGQPPPQD LPGSPKSGPP PEHLPSSPLP
421PHPPSQPCTP ELPAGPRALQ EESLGMEAQV ERAMEGNSSD QEQSPEDEDE GPAEEVPTQM
481PEQNPTHALP ESPRLTLQPK PVVGHFSARE LDGDRSKQLA LKVLCVAQRQ YSLLQAANLL
541TPDMITEFET LQQLVQEEKI EPGAEALRTS GLARGAPLAQ ELCSESKPPG YTGPVTRTMA
601RRLSGPLHTL GIPPGPNCTP AQGSRWPMEK KRRRPSALEA DSPMAPKRGT KRQRQSFLPC
661LRRGSLPDTQ PSQGPSTPKG ERASSPCHSP RVCPATVIKS RVPLGPSAMQ NCSTPLALPT
721RDLNATFDLS EEPPSKPSFH ECIGWDKIPQ ELSRLDQPFI PRAPVPLFTM KGPKPTSSLP
781GTSACKKKRV ASSSVSHGRS RIARLPSSTL KRPAGPLVLP ELPLSPLCPS NRRNGKDLIR
841VGRALSAGNG VTKVS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

855 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.