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KIF18A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIF18A Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIF18A antibodies →
$504.00each
Catalog Number: LS-G101986-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIF18A (NM_031217), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIF18A | Kinesin-like protein KIF18A | Marrow stromal KIF18A | DKFZP434G2226 | Kinesin family member 18A | MS-KIF18A
Origin Species
Human
NCBI GenBank
NM_031217
SwissProt
Q8NI77

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
102.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSVTEEDLCH HMKVVVRVRP ENTKEKAAGF HKVVHVVDKH ILVFDPKQEE VSFFHGKKTT
61NQNVIKKQNK DLKFVFDAVF DETSTQSEVF EHTTKPILRS FLNGYNCTVL AYGATGAGKT
121HTMLGSADEP GVMYLTMLHL YKCMDEIKEE KICSTAVSYL EVYNEQIRDL LVNSGPLAVR
181EDTQKGVVVH GLTLHQPKSS EEILHLLDNG NKNRTQHPTD MNATSSRSHA VFQIYLRQQD
241KTASINQNVR IAKMSLIDLA GSERASTSGA KGTRFVEGTN INRSLLALGN VINALADSKR
301KNQHIPYRNS KLTRLLKDSL GGNCQTIMIA AVSPSSVFYD DTYNTLKYAN RAKDIKSSLK
361SNVLNVNNHI TQYVKICNEQ KAEILLLKEK LKAYEEQKAF TNENDQAKLM ISNPQEKEIE
421RFQEILNCLF QNREEIRQEY LKLEMLLKEN ELKSFYQQQC HKQIEMMCSE DKVEKATGKR
481DHRLAMLKTR RSYLEKRREE ELKQFDENTN WLHRVEKEMG LLSQNGHIPK ELKKDLHCHH
541LHLQNKDLKA QIRHMMDLAC LQEQQHRQTE AVLNALLPTL RKQYCTLKEA GLSNAAFESD
601FKEIEHLVER KKVVVWADQT AEQPKQNDLP GISVLMTFPQ LGPVQPIPCC SSSGGTNLVK
661IPTEKRTRRK LMPSPLKGQH TLKSPPSQSV QLNDSLSKEL QPIVYTPEDC RKAFQNPSTV
721TLMKPSSFTT SFQAVSSNIN SDNCLKMLCE VAIPHNRRKE CGQEDLDSTF TICEDIKSSK
781CKLPEQESLP NDNKDILQRL DPSSFSTKHS MPVPSMVPSY MAMTTAAKRK RKLTSSTSNS
841SLTADVNSGF AKRVRQDNSS EKHLQENKPT MEHKRNICKI NPSMVRKFGR NISKGNLR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

898 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.