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KIF13A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIF13A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110472-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIF13A (NM_022113), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA500C11.2 | Kinesin-like protein RBKIN | KIF13A | RBKIN | Kinesin family member 13A | Kinesin-like protein KIF13A
Origin Species
Human
NCBI GenBank
NM_022113
SwissProt
Q9H1H9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
202.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSDTKVKVAV RVRPMNRREL ELNTKCVVEM EGNQTVLHPP PSNTKQGERK PPKVFAFDYC
61FWSMDESNTT KYAGQEVVFK CLGEGILEKA FQGYNACIFA YGQTGSGKSF SMMGHAEQLG
121LIPRLCCALF KRISLEQNES QTFKVEVSYM EIYNEKVRDL LDPKGSRQSL KVREHKVLGP
181YVDGLSQLAV TSFEDIESLM SEGNKSRTVA ATNMNEESSR SHAVFNIIIT QTLYDLQSGN
241SGEKVSKVSL VDLAGSERVS KTGAAGERLK EGSNINKSLT TLGLVISSLA DQAAGKGKSK
301FVPYRDSVLT WLLKDNLGGN SQTSMIATIS PAADNYEETL STLRYADRAK RIVNHAVVNE
361DPNAKVIREL REEVEKLREQ LSQAEAMKAP ELKEKLEESE KLIKELTVTW EEKLRKTEEI
421AQERQRQLES MGISLEMSGI KVGDDKCYLV NLNADPALNE LLVYYLKDHT RVGADTSQDI
481QLFGIGIQPQ HCEIDIASDG DVTLTPKENA RSCVNGTLVC STTQLWHGDR ILWGNNHFFR
541INLPKRKRRD WLKDFEKETG PPEHDLDAAS EASSEPDYNY EFAQMEVIMK TLNSNDPVQN
601VVQVLEKQYL EEKRSALEEQ RLMYERELEQ LRQQLSPDRQ PQSSGPDRLA YSSQTAQQKV
661TQWAEERDEL FRQGLAKLRE QLVKANTLVR EANFLAEEMS KLTDYQVTLQ IPAANLSANR
721KRGAIVSEPA IQVRRKGKST QVWTIEKLEN KLIDMRDLYQ EWKEKVPEAK RLYGKRGDPF
781YEAQENHNLI GVANVFLECL FCDVKLQYAV PIISQQGEVA GRLHVEVMRV TGAVPERVVE
841DDSSENSSES GSLEVVDSSG EIIHRVKKLT CRVKIKEATG LPLNLSNFVF CQYTFWDQCE
901STVAAPVVDP EVPSPQSKDA QYTVAFSHCK DYVVNVTEEF LEFISDGALA IEVWGHRCAG
961NGSSIWEVDS LHAKTRTLHD RWNEVTRRIE MWISILELNE LGEYAAVELH QAKDVNTGGI
1021FQLRQGHSRR VQVTVKPVQH SGTLPLMVEA ILSVSIGCVT ARSTKLQRGL DSYQRDDEDG
1081DDMDSYQEED LNCVRERWSD ALIKRREYLD EQIKKVSNKT EKTEDDVERE AQLVEQWVGL
1141TEERNAVLVP APGSGIPGAP ADWIPPPGME THIPVLFLDL NADDPSANEQ LVGPHASGVN
1201SILPKEHGSQ FFYLPIIKHS DDEVSATASW DSSVHDSVHL NRVTPQNERI YLIVKTTVQL
1261SHPAAMELVL RKRIAANIYN KQSFTQSLKR RISLKNIFYS CGVTYEIVSN IPKATEEIED
1321RETLALLAAR SENEGTSDGE TYIEKYTRGV LQVENILSLE RLRQAVTVKE ALSTKARHIR
1381RSLSTPNVHN VSSSRPDLSG FDEDDKGWPE NQLDMSDYSS SYQDVACYGT LPRDSPRRNK
1441EGCTSETPHA LTVSPFKAFS PQPPKFFKPL MPVKEEHKKR IALEARPLLS QESMPPPQAH
1501NPGCIVPSGS NGSSMPVEHN NKREKKIDSE EEENELEAIN RKLISSQPYV PVEFADFSVY
1561NASLENREWF SSKVDLSNSR VLEKEVSRSP TTSSITSGYF SHSASNATLS DMVVPSSDSS
1621DQLAIQTKDA DSTEHSTPSL VHDFRPSSNK ELTEVEKGLV KDKIIVVPLK ENSALAKGSP
1681SSQSIPEKNS KSLCRTGSCS ELDACPSKIS QPARGFCPRE VTVEHTTNIL EDHSFTEFMG
1741VSEGKDFDGL TDSSAGELSS RRSLPNKTGG KTVSDGLHHP SQLHSKLEND QVIIPEAAFW
1801VLCCQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1805 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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