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KIF12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIF12 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIF12 antibodies →
$504.00each
Catalog Number: LS-G107388-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIF12 (NM_138424), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIF12 | Kinesin family member 12 | Kinesin-like protein KIF12 | RP11-56P10.3
Origin Species
Human
SwissProt
Q96FN5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MQRTFAWLLD RVQHLGAPVT LRASYLEIYN EQVRDLLSLG SPRPLPVRWN KTRGFYVEQL
61RVVEFGSLEA LMELLQTGLS RRRNSAHTLN QASSRSHALL TLYISRQTAQ QMPSVDPGEP
121PVGGKLCFVD LAGSEKVAAT GSRGELMLEA NSINRSLLAL GHCISLLLDP QRKQSHIPFR
181DSKLTKLLAD SLGGRGVTLM VACVSPSAQC LPETLSTLRY ASRAQRVTTR PQAPKSPVAK
241QPQRLETEML QLQEENRRLQ FQLDQMDCKA SGLSGARVAW AQRNLYGMLQ EFMLENERLR
301KEKSQLQNSR DLAQNEQRIL AQQVHALERR LLSACYHHQQ GPGLTPPCPC LMAPAPPCHA
361LPPLYSCPCC HICPLCRVPL AHWACLPGEH HLPQVLDPEA SGGRPPSARP PPWAPPCSPG
421SAKCPRERSH SDWTQTRVLA EMLTEEEVVP SAPPLPVRPP KTSPGLRGGA GVPNLAQRLE
481ALRDQIGSSL RRGRSQPPCS EGARSPGQVL PPH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

513 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.