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KIAA2013 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIAA2013 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIAA2013 antibodies →
$504.00each
Catalog Number: LS-G107320-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA2013 (NM_138346), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA2013 | RP5-1077B9.1
Origin Species
Human
NCBI GenBank
NM_138346
SwissProt
Q8IYS2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWLQQRLKGL PGLLSSSWAR RLLCLLGLLL LLLWFGGSGA RRAAGGLHLL PWSRGEPGAA
61EPSACLEAAT RAWRGLRERG EVVPLGPGVP ALVANGFLAL DVAANRLWVT PGEREPAVAP
121DFVPFVQLRP LSALAEAGEA VLLLREGLLR RVRCLQLGSP GPGPVAAGPG PASVSGLAAG
181SGRDCVLLQE DFLAHRGRPH VYLQRIQLNN PTERVAALQT VGPTAGPAPK AFTSTLEKVG
241DHQFLLYSGR SPPTPTGLVH LVVVAAKKLV NRLQVAPKTQ LDETVLWVVH VSGPINPQVL
301KSKAAKELKA LQDLARKEML ELLDMPAAEL LQDHQLLWAQ LFSPGVEMKK ITDTHTPSGL
361TVNLTLYYML SCSPAPLLSP SLSHRERDQM ESTLNYEDHC FSGHATMHAE NLWPGRLSSV
421QQILQLSDLW RLTLQKRGCK GLVKVGAPGI LQGMVLSFGG LQFTENHLQF QADPDVLHNS
481YALHGIRYKN DHINLAVLAD AEGKPYLHVS VESRGQPVKI YACKAGCLDE PVELTSAPTG
541HTFSVMVTQP ITPLLYISTD LTHLQDLRHT LHLKAILAHD EHMAQQDPGL PFLFWFSVAS
601LITLFHLFLF KLIYNEYCGP GAKPLFRSKE DPSV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

634 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.