Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIAA1984 / PARF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109807-100
Catalog Number: LS-G109807
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C9orf86(RABL6) (NM_024718), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA216L13.9 | C9orf86 | GTP-binding protein Parf | PARF | Partner of ARF | RBEL1 | Rab-like protein 1 | Pp8875 | RABL6
- Origin Species
- Human
- NCBI GenBank
- NM_024718
- SwissProt
- Q3YEC7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 74.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKIVIRGDRN TGKTALWHRL QGRPFVEEYI PTQEIQVTSI HWSYKTTDDI VKVEVWDVVD61KGKCKKRGDG LKMENDPQEA ESEMALDAEF LDVYKNCNGV VMMFDITKQW TFNYILRELP121KVPTHVPVCV LGNYRDMGEH RVILPDDVRD FIDNLDRPPG SSYFRYAESS MKNSFGLKYL181HKFFNIPFLQ LQRETLLRQL ETNQLDMDAT LEELSVQQET EDQNYGIFLE MMEARSRGHA241SPLAANGQSP SPGSQSPVVP AGAVSTGSSS PGTPQPAPQL PLNAAPPSSV PPVPPSEALP301PPACPSAPAP RRSIISRLFG TSPATEAAPP PPEPVPAAEG PATVQSVEDF VPDDRLDRSF361LEDTTPARDE KKVGAKAAQQ DSDSDGEALG GNPMVAGFQD DVDLEDQPRG SPPLPAGPVP421SQDITLSSEE EAEVAAPTKG PAPAPQQCSE PETKWSSIPA SKPRRGTAPT RTAAPPWPGG481VSVRTGPEKR SSTRPPAEME PGKGEQASSS ESDPEGPIAA QMLSFVMDDP DFESEGSDTQ541RRADDFPVRD DPSDVTDEDE GPAEPPPPPK LPLPAFRLKN DSDLFGLGLE EAGPKESSEE601GKEGKTPSKE KKKKKKKGKE EEEKAAKKKS KHKKSKDKEE GKEERRRRQQ RPPRSRERTA661ADELEAFLGG GAPGGRHPGG GDYEELTRTRPLEQKLISEEDLAANDILDYKDDDDKV
686 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.