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KIAA1958 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIAA1958 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIAA1958 antibodies →
$504.00each
Catalog Number: LS-G107509-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA1958 (NM_133465), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1958
Origin Species
Human
SwissProt
Q8N8K9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEDCLHTSSE NLSKLVSWAH SHGTICSLIP NLKHLLSEGS HGNLTAMWGC SAGHAYHWPL
61TATCRAGSQE RVCFQDNRSF NSDSPSIIGV PSETQTSPVE RYPGRPVKAK LDCNRTRDSC
121DFSYCSEPSE LDETVEEYED ENTLFDMVCE SSVTDEDSDF EPQTQRPQSI ARKRPGVVPS
181SLHSSSQTQM VDECSNDVII KKIKQEIPED YYIVANAELT GGVDGPALSL TQMAKPKPQT
241HAGPSCVGSA KLIPHVTSAI STELDPHGMS ASPSVISRPI VQKTARVSLA SPNRGPPGTH
301GTNQQVAMQM PVSTSHPNKQ ISIPLSALQL PGQDEQVASE EFLSHLPSQV SSCEVALSPS
361VNTEPEVSSS QQQPPVAPAI TTEATAQCIP AYSTKLNKFP VFNINDDLND LCTSAVSPNT
421TKATRYALNV WRYWCMTNGL KDHTDITKIP AVKLNELLEN FYVTVKKSDG SDFLATSLHA
481IRRGLDRILK NAGVGFSITS STFSSSTKKL KEKLWVLSKA GMSGARSRNI VYFSLSDEEE
541MWQAGCLGDD SPITLLSTVV KYNSQYLNMR TLQEHADLMY GDIELLKDPQ NQPYFARTDS
601VKRESRSGST RVCHGKIYHE HSRGHKQCPY CLLYKYMYIH RPPTQMEAKS PFYLTARKEA
661TDMGSVWYEE QRMGLRSLRG IVPNLAKKVK LENCENFTFV SFTQVSRRLG SHSCCQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

716 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.