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KIAA1755 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIAA1755 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIAA1755 antibodies →
$725.00each
Catalog Number: LS-G120798-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA1755 (NM_001029864), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1755 | RP5-1054A22.3
Origin Species
Human
NCBI GenBank
NM_001029864
SwissProt
Q5JYT7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
130.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDPPSLDTAI QHALAGLYPP FEATAPTVLG QVFRLLDSGF QGDGLSFLLD FLIPAKRLCE
61QVREAACAPY SHCLFLHEGW PLCLRDEVVV HLAPLNPLLL RQGDFYLQVE PQEEQSVCIM
121IKCLSLDLCT VDKKPVPEPA YPILFTQEWL EAINSDFEGN PLHNCLVASE NGIAPVPWTK
181ITSPEFVDDR PQVVNALCQA WGPLPLEALD LSSPQELHQA SSPDNQVLPA QSLAKGKGRT
241YGSKYPGLIK VEQARCGEVA FRMDEVVSQD FEGDYVALLG FSQESRGESP SREAGTSSGC
301TSGALEEIAG TKETPLFQKI LPLSEANEGP SLGNRACTKP ESSEERPYNL GFRRKVNLKA
361PTHNSERPPQ GSYMNVLEDA LDCASGLRAG VSQEPAASKM QGPLGNPENM VQLRPGPRQA
421SSPRLSPASP AAAASETKIE VKTKERNGRL PKPMPCPSRN TSSPEPPTPG LKFSFLRGQR
481QPSVTPEKAS LQHNGPWKVL CSLYSPKPNR AKSLGKAGTT QTKTSGPATA PSPLTEEKAA
541LPEASAGSPE RGPTLEEEPP GPEPRIGALG VKVFRSRIAC LPGGRDRAGR PLLLVSTTEG
601AWEAPWCTVS EVTKLLSYLC TIPRPEDKAK GLAVLIDARR QPPQPGLVSA LQATQAQVPA
661SIRAILFLGE KEAALQLQTL PDVQVEVLTS LKALSHHVDP SQLPAVLEGP FPYCHTEWVH
721FFQKLDPFLA DLHQASSLLQ ASIEEFEKAD PPGGMQEATR CLSKSKELME AVLRDPGLLG
781LQREGGATLA RLQHDASRLD FSPDVRSHLA AATALYSLVD EQLHVLVTAS NSLLGKLELR
841VRLGRLEAAI HQVSDWMEQE GRRCLQSLTP KDGSLETVEK AHAEFENFFL QAAAQYRRGL
901ELSKQAAQLG ATARGAGEAE RAEFPELAAF ASTQRAFQAE LTHFYMAAER QRTDLETLLH
961LHRFCKRMTW FHMDCQDLMA QLRLDKTSRV SPGDQRRLHR YLQRLASEFP AEKLAAVGLQ
1021VASLSRAGLG QELWEEARIR HEEIRMLLEK ALTHSSCPEA PAAHSARPER RGVAAKGQGV
1081SVEVTSKGRW DQPPLDSLGM DHLPKSYWPP GPPRGEQNRT FQAGSPPQEA GQAAEAEDGK
1141GSHKLPDPAR EHLLATTFFR QQPPRQSQVP RLTGGSFSSE GTDSQTSLED SPQTSPLASL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1200 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.