Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIAA1754L / ITPRIPL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104635-100
Catalog Number: LS-G104635
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ITPRIPL1 (NM_178495), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ITPRIPL1 | KIAA1754L | KIAA1754-like
- Origin Species
- Human
- NCBI GenBank
- NM_178495
- SwissProt
- Q6GPH6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTTDSYSPTS PDAEASMAVI SLLFLAVMYV VHHPLMVSDR MDLDTLARSR QLEKRMSEEM61RLLEMEFEER KRAAEQRQKA ENFWTGDTSS DQLVLGKKDM GWPFQADGQE GPLGWMLGNL121WNTGLFCLFL VFELLRQNMQ HEPAFDSSSE EEEEVRVVPV TSYNWLTDFP SQEALDSFYK181HYVQNAIRDL PCTCEFVESF VDDLIEACRV LSRQEAHPQL EDCLGIGAAF EKWGTLHETQ241KFDILVPIVP PQGTMFVLEM RDPALGRRCG CVLVESECVC KREKLLRDVL CLVHHHRDPS301AVLGKCSSSI KAALCTGFHL DVCKTVQWFR NMMGNAWALV AHKYDFKLSL PPSTTSCKLR361LDYRSGRFLS IHLVLGVQRE DTLVYLVSQA PDQEQLTSVD WPESFVACEH LFLKLVGRFA421PENTCHLKCL QIILSLRQHQ SLPHGASRPI LTSYHFKTAL MHLLLRLPLT DWAHNMLSQR481LQDILWFLGR GLQQRSLHHF LIGNNFLPLT IPIPKTFRNA EPVNLFQHLV LNPKAHSQAV541EEFQNLLTQV KTLPHAPLAA APTRTRPLEQKLISEEDLAANDILDYKDDDDKV
562 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.