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KIAA1614 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIAA1614 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIAA1614 antibodies →
$725.00each
Catalog Number: LS-G110861-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA1614 (NM_020950), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_020950

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
126.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEGTEAAAAK PAGGSPQGPK TGSGTASPVE GTSAVEWSGP EPQLDNGHPP RPWPCPQENR
61TSSLMAPQPP RVWGVQLQGP SVLESKVRAL KEKMTVAKQG VSPCSASQEW SSPKKPQCRR
121GKAGRAGTPS EGSFLPGAVV APRTQNLPDG QLDGSINEEQ PARDGGPRLP RPPAPGREYC
181NRGSPWPPEA EWTLPDHDRG PLLGPSSLQQ SPIHGVTPGR PGGPGHCNKI IHIPSPRTGR
241SYPFPDGVVT EADLDSTSLT SEEVFVPRTA LLGERWRAGD LEALGAGSSV LSLSDRVERN
301RLLLQEMLNV SGQSPRKVGT PAWTPSWDTA APERPVGDVD WASGTSLQDS GQNRTVGPNP
361EPVLSPRHEE ATHLLQRARM KARTRPLRAS HDIVPTITQG SRDGHRSPAR DPRTTPACRD
421SLQNGHTSDS SSGESSGGHR PRRGPSPSHV RFEDESAREA EFRHLERLQQ RQRQVLSTVL
481QAADQGPLRS KPDLADYING APRLRDAGQG TFHRLVGSLD RRGHPAPPAP GSERRCQACG
541SCIDDPRPAQ GKAPPVPRTL QELQAACGME RVLGGLSSPL RLLPAEPRLH MEWIRETHIG
601DTVCPAEVDS ALDSTDNSDN CRTDSEEAGT SQAGWACGRT QGSSPRLRLR GSRPRGHRWS
661KKAEAELPWG LQAQQHLPRA DDVEVENEVK EGRGHTPEGT LFLREDAKPP DLELKRVSLG
721PQWQPGPGLG SHQPHPLDSR TPCRTAYATT APMTPESSGP GGQAQVTESH ESLEIVSPSS
781LQQSHAEPSA PHQAWQPTAS LCPEGWAPTP PPSRKTTSPV SHRKAALAGL LRLGDQTEPV
841GIPRPPSRSA VLRTCELPPS QTQPSRPQVR HPLLALSTNN CNNSAPRGLQ EPYGGAVHEG
901RVERGPCSRE PEPPLENSRD GGPQGFLGSA DVATINSTGI TLSLSSEESE SSKESEGSLQ
961RTGSGSGGHV LSRASAGAGT GPGSPSAAPL DQNKKRSSSI ASTLGLKKLF SALGQSSRPK
1021LGKSRSYSVE QLQPAPPGLT SQSRAPSLQS LHPVSPSHQR RKAASFQNLH SLLSSKGNRS
1081SLYLVAGPGD HSAAGRPAKT SPRRALSVED VGAPSLARTV GRLVEVFPDG TSQLQLQRSP
1141GGTFGFCVAS GNGRPDSGMP SPLPQPHGWG GLSKQGRAFW LWSEAFLVFG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1190 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.