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KIAA1586 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIAA1586 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIAA1586 antibodies →
$504.00each
Catalog Number: LS-G110912-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA1586 (NM_020931), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1586
Origin Species
Human
NCBI GenBank
AB046806
SwissProt
Q9HCI6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGDPGSEIIE SVPPAGPEAS ESTTDENEDD IQFVSEGPSR PVLEYIDLVC GDDENPSAYY
61SDILFPKMPK RQGDFLHFLN VKKVKTDTEN NEVSKNHCRL SKAKEPHFEY IEQPIIEEKP
121SLSSKKEIDN LVLPDCWNEK QAFMFTEQYK WLEIKEGKLG CKDCSAVRHL GSKAEKHVHV
181SKEWIAYLVT PNGSNKTTRQ ASLRKKIREH DVSKAHGKIQ DLLKESTNDS ICNLVHKQNN
241KNIDATVKVF NTVYSLVKHN RPLSDIEGAR ELQEKNGEVN CLNTRYSATR IAEHIAKEMK
301MKIFKNIIEE NAKICIIIDE ASTVSKKTTL VIYLQCTIQS APAPVMLFVA LKELVSTIAE
361CIVNTLLTTL NDCGFTNEYL KANLIAFCSD GANTILGRKS GVATKLLENF PEIIIWNCLN
421HRLQLSLDDS ISEIKQINHL KIFIDKIYSI YHQPNKNQTK LLGTVAKELE TEIIKIGRVM
481GPRWAACSLQ AATAVWHAYP ILYMHFSHSY SGLAKRLANI NFLQDLALMI DILEEFSVLS
541TALQSRSTNI KKAQKLIKRT IRALENLKIG TGKYESQIED LIKSDKFKDI PFNKNNKFNA
601LPRSILLDNI IQHMNLRLLS DRNHEDIFNY FDLLEPSTWP YEEITSPWIA GEKTLFHLCK
661ILKYEVDLND FREFVNNNIK SNNVSIPTTI YKAKKIVSTI AINSAEAERG FNLMNIICTR
721VRNSLTIDHV SDLMTINLLG KELADWDATP FVKSWSNCNH RLATDTRVRQ KSTKVFHENQ
781LAIWNLK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

787 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.