Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIAA1524 / p90 Autoantigen Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110936-100
Catalog Number: LS-G110936
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human p90 Autoantigen(KIAA1524) (NM_020890), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cancerous inhibitor of PP2A | CIP2A | Protein CIP2A | KIAA1524 | p90 autoantigen | p90
- Origin Species
- Human
- NCBI GenBank
- AB040957
- SwissProt
- Q8TCG1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 102 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSTACLKSL LLTVSQYKAV KSEANATQLL RHLEVISGQK LTRLFTSNQI LTSECLSCLV61ELLEDPNISA SLILSIIGLL SQLAVDIETR DCLQNTYNLN SVLAGVVCRS SHTDSVFLQC121IQLLQKLTYN VKIFYSGANI DELITFLIDH IQSSEDELKM PCLGLLANLC RHNLSVQTHI181KTLSNVKSFY RTLITLLAHS SLTVVVFALS ILSSLTLNEE VGEKLFHARN IHQTFQLIFN241ILINGDGTLT RKYSVDLLMD LLKNPKIADY LTRYEHFSSC LHQVLGLLNG KDPDSSSKVL301ELLLAFCSVT QLRHMLTQMM FEQSPPGSAT LGSHTKCLEP TVALLRWLSQ PLDGSENCSV361LALELFKEIF EDVIDAANCS SADRFVTLLL PTILDQLQFT EQNLDEALTR KKCERIAKAI421EVLLTLCGDD TLKMHIAKIL TTVKCTTLIE QQFTYGKIDL GFGTKVADSE LCKLAADVIL481KTLDLINKLK PLVPGMEVSF YKILQDPRLI TPLAFALTSD NREQVQSGLR ILLEAAPLPD541FPALVLGESI AANNAYRQQE TEHIPRKMPW QSSNHSFPTS IKCLTPHLKD GVPGLNIEEL601IEKLQSGMVV KDQICDVRIS DIMDVYEMKL STLASKESRL QDLLETKALA LAQADRLIAQ661HRCQRTQAET EARTLASMLR EVERKNEELS VLLKAQQVES ERAQSDIEHL FQHNRKLESV721AEEHEILTKS YMELLQRNES TEKKNKDLQI TCDSLNKQIE TVKKLNESLK EQNEKSIAQL781IEKEEQRKEV QNQLVDREHK LANLHQKTKV QEEKIKTLQK EREDKEETID ILRKELSRTE841QIRKELSIKA SSLEVQKAQL EGRLEEKESL VKLQQEELNK HSHMIAMIHS LSGGKINPET901VNLSITRTRPLEQKLISEEDLAANDILDYKDDDDKV
905 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.