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KIAA1468 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIAA1468 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIAA1468 antibodies →
$725.00each
Catalog Number: LS-G110957-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA1468 (NM_020854), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
134.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAMAPGGSG SGGGVNPFLS DSDEDDDEVA ATEERRAVLR LGAGSGLDPG SAGSLSPQDP
61VALGSSARPG LPGEASAAAV ALGGTGETPA RLSIDAIAAQ LLRDQYLLTA LELHTELLES
121GRELPRLRDY FSNPGNFERQ SGTPPGMGAP GVPGAAGVGG AGGREPSTAS GGGQLNRAGS
181ISTLDSLDFA RYSDDGNRET DEKVAVLEFE LRKAKETIQA LRANLTKAAE HEVPLQERKN
241YKSSPEIQEP IKPLEKRALN FLVNEFLLKN NYKLTSITFS DENDDQDFEL WDDVGLNIPK
301PPDLLQLYRD FGNHQVTGKD LVDVASGVEE DELEALTPII SNLPPTLETP QPAENSMLVQ
361KLEDKISLLN SEKWSLMEQI RRLKSEMDFL KNEHFAIPAV CDSVQPPLDQ LPHKDSEDSG
421QHPDVNSSDK GKNTDIHLSI SDEADSTIPK ENSPNSFPRR EREGMPPSSL SSKKTVHFDK
481PNRKLSPAFH QALLSFCRMS ADSRLGYEVS RIADSEKSVM LMLGRCLPHI VPNVLLAKRE
541ELIPLILCTA CLHPEPKERD QLLHILFNLI KRPDDEQRQM ILTGCVAFAR HVGPTRVEAE
601LLPQCWEQIN HKYPERRLLV AESCGALAPY LPKEIRSSLV LSMLQQMLME DKADLVREAV
661IKSLGIIMGY IDDPDKYHQG FELLLSALGD PSERVVSATH QVFLPAYAAW TTELGNLQSH
721LILTLLNKIE KLLREGEHGL DEHKLHMYLS ALQSLIPSLF ALVLQNAPFS SKAKLHGEVP
781QIEVTRFPRP MSPLQDVSTI IGSREQLAVL LQLYDYQLEQ EGTTGWESLL WVVNQLLPQL
841IEIVGKINVT STACVHEFSR FFWRLCRTFG KIFTNTKVKP QFQEILRLSE ENIDSSAGNG
901VLTKATVPIY ATGVLTCYIQ EEDRKLLVGF LEDVMTLLSL SHAPLDSLKA SFVELGANPA
961YHELLLTVLW YGVVHTSALV RCTAARMFEL TLRGMSEALV DKRVAPALVT LSSDPEFSVR
1021IATIPAFGTI METVIQRELL ERVKMQLASF LEDPQYQDQH SLHTEIIKTF GRVGPNAEPR
1081FRDEFVIPHL HKLALVNNLQ IVDSKRLDIA THLFEAYSAL SCCFISEDLM VNHFLPGLRC
1141LRTDMEHLSP EHEVILSSMI KECEQKVENK TVQEPQGSMS IAASLVSEDT KTKFLNKMGQ
1201LTTSGAMLAN VFQRKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1216 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.