Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIAA1456 / C8orf79 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110950-100
Catalog Number: LS-G110950
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA1456 (NM_020844), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C8orf79 | KIAA1456
- Origin Species
- Human
- NCBI GenBank
- NM_001099677
- SwissProt
- Q9P272
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDHEAAQLEK QHVHNVYEST APYFSDLQSK AWPRVRQFLQ EQKPGSLIAD IGCGTGKYLK61VNSQVHTVGC DYCGPLVEIA RNRGCEAMVC DNLNLPFRDE GFDAIISIGV IHHFSTKQRR121IRAIKEMARV LVPGGQLMIY VWAMEQKNRR FEKQDVLVPW NRALCSQLFS ESSQSGRKRQ181CGYPERGHPY HPPCSECSCS VCFKEQGGSK RSHSVGYEPA MARTCFANIS KEGEEEYGFY241STLGKSFRSW FFSRSLDEST LRKQIERVRP LKNTEVWASS TVTVQPSRHS SLDFDHQEPF301STKEQSLDEE VFVESSSGKH LEWLRAPGTL KHLNGDHQGE MRRNGGGNFL DSTNTGVNCV361DAGNIEDDNP SASKILRRIS AVDSTDFNPD DTMSVEDPQT DVLDSTAFMR YYHVFREGEL421CSLLKENVSE LRILSSGNDH GNWCIIAEKK RGCDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
454 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.