LSBio
KIAA1328 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIAA1328 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIAA1328 antibodies →
$725.00each
Catalog Number: LS-G111015-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA1328 (NM_020776), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Hinderin | KIAA1328
Origin Species
Human
NCBI GenBank
AB037749
SwissProt
Q86T90

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADVAGPSRP SAAAFWSRDF SDEEQSVVYV PGISAEGNVR SRHKLMSPKA DVKLKTSRVT
61DASISMESLK GTGDSVDEQN SCRGEIKSAS LKDLCLEDKR RIANLIKELA RVSEEKEVTE
121ERLKAEQESF EKKIRQLEEQ NELIIKEREA LQLQYRECQE LLSLYQKYLS EQQEKLTMSL
181SELGAARMQE QQVSSRKSTL QCSSVELDGS YLSIARPQTY YQTKQRPKSA VQDSASESLI
241AFRNNSLKPV TLHHPKDDLD KIPSETTTCN CESPGRKPAV PTEKMPQEEL HMKECPHLKP
301TPSQCCGHRL AADRVHDSHP TNMTPQHPKT HPESCSYCRL SWASLVHGGG ALQPIETLKK
361QISEDRKQQL MLQKMELEIE KERLQHLLAQ QETKLLLKQQ QLHQSRLDYN CLLKSNCDGW
421LLGTSSSIKK HQDPPNSGEN RKERKTVGFH SHMKDDAQWS CQKKDTCRPQ RGTVTGVRKD
481ASTSPMPTGS LKDFVTTASP SLQHTTSRYE TSLLDLVQSL SPNSAPKPQR YPSREAGAWN
541HGTFRLSPLK STRKKMGMHR TPEELEENQI LEDIFFI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

577 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.