Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
KIAA1276 / FAM184B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G127095-100
Catalog Number: LS-G127095
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA1276(FAM184B) (NM_015688), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FAM184B | KIAA1276 | Protein FAM184B
- Origin Species
- Human
- NCBI GenBank
- AB033102
- SwissProt
- Q9ULE4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 120.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASALNSKIN PPGTCQGSKA DGGAGWRMDC DPQMHVKMCK KIAQLTKVIY ALNTRQDEAE61ASMEALREAH QEELQNAVAE TKARLLQEQG CAEEEALLQR IQALESALEL QKRLTEEALA121ESASCRLETK ERELRVEAEH AERVLTLSRE MLELKADYER RLQHLTSHEA TPQGRLPQES181PETKSEPGQG PEMQEVLLEV QRLRVENQQL SKDYARKAEE LQATYERENE AIRQAMQQSV241SQALWQWQEK ESDLRKNFQV QESALQAQVR KLEGDLEHRG RKISDLKKYA QKLKERIQDL301DVQLKEARQE NSELKGTAKK LGEKLAVAKD RMMLQECRGT QQTDAMKTEL VSENKVLREE361NDLEAGNLHP QQDQSCLKEC PCMKGGTDMQ TKKEASAETE YMKQQYEEDL RKIKHQTEEE421KKHLKDQLVK RLEDLVKKHT VEIKSVRSSV EAERKKLQRE VEAQLEEVRK KSEKEIKQLE481EEKAALNVKL QNSLLEVLRL EEFIQQNKTR PTGAEESPQE LGRQHCSILE TQDPCLKLDE541TSPRGEEYQD KLAAEEGTSS DEEERTKVLL KEGSDPQPPL GSLLKEKTSK IQRLEEDWQS601QKAKLQAQVS QMQQALEQCT SNYREDLQAL KQLSDLEREK LQRELQETTQ QNHAMKAQLE661ASHQRALRML EKARHQELKA TEERLKKESS HSLQIQHQTH RLELQALEEK ARQELQEERE721RMQAQQALLL ESLRQELSEQ QAACSGHQKD LEALQAELRA LGRQQASSQC PGDSKDHIIA781TEERGGPGQA GSPPGAAGQG SGEGCGLWEE NAQLQDAVRR LRAEVEQHQQ EAQKLRDQRR841FLEETQQAQR AREVETLRQE HRKEMQAMVA DFSSAQAQLQ ARLAALEAEL KDSGEKPGKG901ASRPEDLQLI GRLQTRLKER EDIIKQLTEE RRFHYAAFPS AMSHRNRSFS FNPHPGYLTP961SMKKKKVEDV PSRVVSVPNL ASYAKNFLSG DLSSRINAPP ITTSPSLDPS PSCGRTYKPN1021QSTDAKTATR TPDGETAQAK EVQQKQGSPH QEWFTKYFSFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1060 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.