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KIAA1217 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIAA1217 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIAA1217 antibodies →
$725.00each
Catalog Number: LS-G111383-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SKT(KIAA1217) (NM_019590), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_019590

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
213.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEENESQKCE PCLPYSADRR QMQEQGKGNL HVTSPEDAEC RRTKERLSNG NSRGSVSKSS
61RNIPRRHTLG GPRSSKEILG MQTSEMDRKR EAFLEHLKQK YPHHASAIMG HQERLRDQTR
121SPKLSHSPQP PSLGDPVEHL SETSADSLEA MSEGDAPTPF SRGSRTRASL PVVRSTNQTK
181ERSLGVLYLQ YGDETKQLRM PNEITSADTI RALFVSAFPQ QLTMKMLESP SVAIYIKDES
241RNVYYELNDV RNIQDRSLLK VYNKDPAHAF NHTPKTMNGD MRMQRELVYA RGDGPGAPRP
301GSTAHPPHAI PNSPPSTPVP HSMPPSPSRI PYGGTRSMVV PGNATIPRDR ISSLPVSRPI
361SPSPSAILER RDVKPDEDMS GKNIAMYRNE GFYADPYLYH EGRMSIASSH GGHPLDVPDH
421IIAYHRTAIR SASAYCNPSM QAEMHMEQSL YRQKSRKYPD SHLPTLGSKT PPASPHRVSD
481LRMIDMHAHY NAHGPPHTMQ PDRASPSRQA FKKEPGTLVY IEKPRSAAGL SSLVDLGPPL
541MEKQVFAYST ATIPKDRETR ERMQAMEKQI ASLTGLVQSA LFKGPITSYS KDASSEKMMK
601TTANRNHTDS AGTPHVSGGK MLSALESTVP PSQPPPVGTS AIHMSLLEMR RSVAELRLQL
661QQMRQLQLQN QELLRAMMKK AELEISGKVM ETMKRLEDPV QRQRVLVEQE RQKYLHEEEK
721IVKKLCELED FVEDLKKDST AASRLVTLKD VEDGAFLLRQ VGEAVATLKG EFPTLQNKMR
781AILRIEVEAV RFLKEEPHKL DSLLKRVRSM TDVLTMLRRH VTDGLLKGTD AAQAAQYMAM
841EKATAAEVLK SQEEAAHTSG QPFHSTGAPG DAKSEVVPLS GMMVRHAQSS PVVIQPSQHS
901VALLNPAQNL PHVASSPAVP QEATSTLQMS QAPQSPQIPM NGSAMQSLFI EEIHSVSAKN
961RAVSIEKAEK KWEEKRQNLD HYNGKEFEKL LEEAQANIMK SIPNLEMPPA TGPLPRGDAP
1021VDKVELSEDS PNSEQDLEKL GGKSPPPPPP PPRRSYLPGS GLTTTRSGDV VYTGRKENIT
1081AKASSEDAGP SPQTRATKYP AEEPASAWTP SPPPVTTSSS KDEEEEEEEG DKIMAELQAF
1141QKCSFMDVNS NSHAEPSRAD SHVKDTRSGA TVPPKEKKNL EFFHEDVRKS DVEYENGPQM
1201EFQKVTTGAV RPSDPPKWER GMENSISDAS RTSEYKTEII MKENSISNMS LLRDSRNYSQ
1261ETVPKASFGF SGISPLEDEI NKGSKISGLQ YSIPDTENQT LNYGKTKEME KQNTDKCHVS
1321SHTRLTESSV HDFKTEDQEV ITTDFGQVVL RPKEARHANV NPNEDGESSS SSPTEENAAT
1381DNIAFMITET TVQVLSSGEV HDIVSQKGED IQTVNIDARK EMTPRQEGTD NEDPVVCLDK
1441KPVIIIFDEP MDIRSAYKRL STIFEECDEE LERMMMEEKI EEEEEEENGD SVVQNNNTSQ
1501MSHKKVAPGN LRTGQQVETK SQPHSLATET RNPGGQEMNR TELNKFSHVD SPNSECKGED
1561ATDDQFESPK KKFKFKFPKK QLAALTQAIR TGTKTGKKTL QVVVYEEEEE DGTLKQHKEA
1621KRFEIARSQP EDTPENTVRR QEQPSIESTS PISRTDEIRK NTYRTLDSLE QTIKQLENTI
1681SEMSPKALVD TSCSSNRDSV ASSSHIAQEA SPRPLLVPDE GPTALEPPTS IPSASRKGSS
1741GAPQTSRMPV PMSAKNRPGT LDKPGKQSKL QDPRQYRQAN GSAKKSGGDF KPTSPSLPAS
1801KIPALSPSSG KSSSLPSSSG DSSNLPNPPA TKPSIASNPL SPQTGPPAHS ASLIPSVSNG
1861SLKFQSLTHT GKGHHLSFSP QSQNGRAPPP LSFSSSPPSP ASSVSLNQGA KGTRTIHTPS
1921LTSYKAQNGS SSKATPSTAK ETS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1943 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.