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KIAA1033 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

KIAA1033 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all KIAA1033 antibodies →
$725.00each
Catalog Number: LS-G113335-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WASH complex subunit 7(KIAA1033) (NM_015275), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
WASH complex subunit 7 | SWIP | KIAA1033
Origin Species
Human
NCBI GenBank
AB028956
SwissProt
Q2M389

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
136.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVETLSPDW EFDRVDDGSQ KIHAEVQLKN YGKFLEEYTS QLRRIEDALD DSIGDVWDFN
61LDPIALKLLP YEQSSLLELI KTENKVLNKV ITVYAALCCE IKKLKYEAET KFYNGLLFYG
121EGATDASMVE GDCQIQMGRF ISFLQELSCF VTRCYEVVMN VVHQLAALYI SNKIAPKIIE
181TTGVHFQTMY EHLGELLTVL LTLDEIIDNH ITLKDHWTMY KRLLKSVHHN PSKFGIQEEK
241LKPFEKFLLK LEGQLLDGMI FQACIEQQFD SLNGGVSVSK NSTFAEEFAH SIRSIFANVE
301AKLGEPSEID QRDKYVGICG LFVLHFQIFR TIDKKFYKSL LDICKKVPAI TLTANIIWFP
361DNFLIQKIPA AAKLLDRKSL QAIKIHRDTF LQQKAQSLTK DVQSYYVFVS SWMMKMESIL
421SKEQRMDKFA EDLTNRCNVF IQGFLYAYSI STIIKTTMNL YMSMQKPMTK TSVKALCRLV
481ELLKAIEHMF YRRSMVVADS VSHITQHLQH QALHSISVAK KRVISDKKYS EQRLDVLSAL
541VLAENTLNGP STKQRRLIVS LALSVGTQMK TFKDEELFPL QVVMKKLDLI SELRERVQTQ
601CDCCFLYWHR AVFPIYLDDV YENAVDAARL HYMFSALRDC VPAMMHARHL ESYEILLDCY
661DKEIMEILNE HLLDKLCKEI EKDLRLSVHT HLKLDDRNPF KVGMKDLALF FSLNPIRFFN
721RFIDIRAYVT HYLDKTFYNL TTVALHDWAT YSEMRNLATQ RYGLVMTEAH LPSQTLEQGL
781DVLEIMRNIH IFVSRYLYNL NNQIFIERTS NNKHLNTINI RHIANSIRTH GTGIMNTTVN
841FTYQFLKKKF YIFSQFMYDE HIKSRLIKDI RFFREIKDQN DHKYPFDRAE KFNRGIRKLG
901ITPEGQSYLD QFRQLISQIG NAMGYVRMIR SGGLHCSSNA IRFVPDLEDI VNFEELVKEE
961GLAEETLKAA RHLDSVLSDH TRNSAEGTEY FKMLVDVFAP EFRRPKNIHL RNFYIIVPPL
1021TLNFVEHSIS CKEKLNKKNK IGAAFTDDGF AMGVAYILKL LDQYREFDSL HWFQSVREKY
1081LKEIRAVAKQ QNVQSASQDE KLLQTMNLTQ KRLDVYLQEF ELLYFSLSSA RIFFRADKTA
1141AEENQEKKEK EEETKTSNGD LSDSTVSADP VVK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1173 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.